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用生物学和血清学方法从苹果分离鉴定出苹果茎沟病毒G-2和TC-3分离物,采用皂土澄清、15％蔗糖垫超离心、10％～40％蔗糖梯度离心等步骤,获得提纯的病毒样品,紫外吸收比值A_(260/280)为1.15。13％SDS-PAGE法测定的病毒外壳蛋白分子量为31000。用提纯的苹果茎沟病毒G-2分离物制备的兔抗血清,PTA-ELISA测定的效价为1/64000,特异性强。用PAS-ELISA可成功地检测出苹果茎沟病毒,此外还试验了DAS-ELISA,并证明其检测苹果茎沟病毒的效果与PAS-ELISA一致。
Isolation and identification of G-2 and TC-3 isolates from apple using biological and serological methods were performed using the steps of clarification of bentonite, ultracentrifugation with 15% sucrose and centrifugation of 10% -40% sucrose to obtain Purified virus samples, UV absorbance ratio A 260-280 1.15.13% SDS-PAGE assay of the viral coat protein molecular weight of 31,000. Rabbit antisera prepared from purified apple stem gavage virus G-2 isolate showed a titer of 1/64000 as measured by PTA-ELISA with high specificity. The PAS-ELISA successfully detected the apple stem gully virus. In addition, the DAS-ELISA was also tested. The results showed that the PAS-ELISA was consistent with PAS-ELISA.