The effects of tempering holding time at 700℃ on the morphology, mechanical properties, and behavior of nanoparticles in Ti-Mo ferritic steel with different Mo contents were analyzed using scanning electron microscopy and transmission electron microscopy. The equilib-rium solid solution amounts of Mo, Ti, and C in ferritic steel at various temperatures were calculated, and changes in the sizes of nanoparticles over time at different Mo contents were analyzed. The experimental results and theoretical calculations were in good agreement with each oth-er and showed that the size of nanoparticles in middle Mo content nano-ferrite (MNF) steel changed the least during aging. High Mo contents inhibited the maturation and growth of nanoparticles, but no obvious inhibitory effect was observed when the Mo content exceeded 0.37wt％. The tensile strength and yield strength continuously decreased with the tempering time. Analysis of the strengthening and toughening mechan-isms showed that the different mechanical properties among the three different Mo content experiment steels were mainly determined by grain refinement strengthening (the difference range was 30-40 MPa) and precipitation strengthening (the difference range was 78-127 MPa). MNF steel displayed an ideal chemical ratio and the highest thermodynamic stability, whereas low Mo content nano-ferrite (LNF) steel and high Mo content nano-ferrite (HNF) steel displayed relatively similar thermodynamic stabilities.