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目的观察大蒜素(Gar)对H9c2细胞钠电流的影响。方法取培养大鼠心肌细胞系H9c2细胞,采用全细胞膜片钳记录钠电流。观察Gar对钠电流和门控机制的影响。结果 Gar对I_(Na)的抑制效应呈电压依赖性。-30 m V时,200μmol/L Gar可使I_(Na)由(-98.4±5.6)pA/pF降低为(-66.5±5.3)pA/pF(P<0.01)。药物使I_(Na)半激活电压V_(1/2)向去极化方向移动,减少通道开放,从而减少I_(Na)电流峰值密度。200μmol/L的Gar可以使钠通道的稳态激活曲线向去极化方向移动,半激活电压(V_(1/2),act)从(-50.6±5.4)m V移至(-27.8±3.2)m V(P<0.01),激活曲线斜率(kact)差异无统计学意义(P>0.05)。结论大蒜素可能通过减少钠通道激活,降低钠电流。
Objective To observe the effect of Gar on sodium current in H9c2 cells. Methods H9c2 cells were cultured in rat cardiomyocytes. Whole-cell patch-clamp was used to record sodium current. The effect of Gar on sodium current and gating mechanism was observed. As a result, the inhibitory effect of Gar on I_ (Na) was voltage-dependent. At -30 mV, 200μmol / L Gar reduced I Na from (-98.4 ± 5.6) pA / pF to -66.5 ± 5.3 pA / pF (P <0.01). The drug moves the I_ (Na) half activation voltage V_ (1/2) to the depolarization direction, reduces the channel opening, and reduces the peak current density of I_ (Na). 200μmol / L Gar could shift the steady-state activation curve of sodium channels toward depolarization and the half activation voltage (V 1/2, act) shifted from (-50.6 ± 5.4) m V to (-27.8 ± 3.2 ) m V (P <0.01). There was no significant difference in the activation curve slope (kact) (P> 0.05). Conclusion Allicin may decrease sodium current by reducing sodium channel activation.