论文部分内容阅读
以低磷胁迫和平衡施肥处理的番茄根系及根际土壤为研究对象,采用Win RHIZO根系分析系统、稀释平板法、PCR-DGGE等分析技术,研究低磷胁迫对番茄根系生长、根际土壤生物学特性以及细菌多样性的影响。结果表明:低磷胁迫导致番茄根系总根长、总根表面积、总根体积和根尖数减少;导致根际土壤中可培养微生物(细菌、真菌和放线菌)数量、酶活性、微生物生物量(C、N、P)以及细菌的多样性指数(H)、丰富度(S)和均匀度指数(Eh)等表征土壤肥力及健康状态的指标下降;导致部分诸如甲基杆菌属(Methylobacterium sp.)等具有溶磷功能的菌群缺失;平衡施肥对番茄的正常生长和提升土壤肥力以及维护土壤健康具有极其重要的作用。
The root system and rhizosphere soil of tomato were treated with low-phosphorus stress and balanced fertilization. The effects of low-phosphorus stress on the growth of root system, rhizosphere soil and soil were studied by using Win RHIZO root system, dilution plate method and PCR-DGGE. Learning characteristics and the impact of bacterial diversity. The results showed that the total root length, the total root surface area, the total root volume and the number of root tips of tomato root system were decreased under low-phosphorus stress. The number of rhizosphere soil microorganisms (bacteria, fungi and actinomycetes), enzyme activity, microbial biomass (C, N, P) as well as bacterial diversity index (H), richness (S) and evenness index (Eh) and other indicators of soil fertility and health status indicators; led to such as Methylobacterium sp.) and other bacteria with phosphate-solubilizing function were absent. Balanced fertilization played an important role in the normal growth of tomato and soil fertility as well as the maintenance of soil health.