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植物钾(K+)转运体蛋白在 K+的跨膜运输中起重要作用,进而维持植物体正常生长和代谢活动。本研究中,通过隐马尔科夫模型(HMM)和葡萄蛋白质库搜索,共找到 18 个葡萄钾转运体蛋白(VvKUPs)。利用生物信息学方法,我们对葡萄家族 12 条 KUP 蛋白序列的系统发生和 KUP 基因组定位进行分析,然后对其氨基酸组成成分、理化性质以及二级结构进行预测和分析,同时还分析了葡萄与拟南芥、水稻和杨树的 KUP 基因家族之间的联系。基因组定位结果发现其分布在至少 9 条染色体上。二级结构预测结果发现不同成员间氨基酸数目、氨基酸序列间的疏水性存在一定的差异;α- 螺旋和无规则卷曲为主要二级结构组成部分。基因结构分析表明,KUP 基因家族成员分别含有 7~10 个内含子。葡萄 KUP 蛋白的亚细胞定位分析表明 VvKUP 主要定位于膜结构上。电子表达图谱分析结果表明:12 条 KUP 基因有对应的 EST 序列,其中的 11 条 KUP 有相应的电子表达谱,并主要在花、果实、花序和花蕾等组织部位表达。
Plant potassium (K +) transporter proteins play an important role in the transmembrane transport of K +, thus maintaining normal plant growth and metabolic activity. In this study, 18 grape potassium transporter proteins (VvKUPs) were found by Hidden Markov Model (HMM) and grape protein library search. Using bioinformatics methods, we analyzed the phylogeny and KUP genomic mapping of 12 KUP proteins in grape families, then predicted and analyzed their amino acid composition, physicochemical properties and secondary structure. Arabidopsis, rice and poplar KUP gene family linkages. Genome localization results were found in at least nine chromosomes. The results of secondary structure prediction showed that the number of amino acids and the hydrophobicity of amino acid sequences among different members were different. The α-helix and random coil were the main secondary structural components. Gene structure analysis showed that KUP gene family members contain 7 to 10 introns respectively. Subcellular localization of KUP protein in grape showed that VvKUP mainly localized on the membrane structure. The results of electronic expression profiling showed that 12 KUP genes had corresponding EST sequences, of which 11 KUP had corresponding electronic expression profiles and were mainly expressed in floral, fruit, inflorescence and flower buds.