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背景:乳腺癌易感蛋白2(breastcancersusceptibilityprotein2,BRCA2)是由乳腺癌易感基因2编码的一种在维持哺乳动物细胞染色体稳定及DNA损伤生物应答中发挥重要作用的肿瘤抑制因子。许多研究表明:BRCA2蛋白与遗传性乳腺癌、卵巢癌及范康尼氏贫血症的发生具有密切关系,猜测其与双链DNA损伤修复有关,但具体作用模型与机制仍不清楚。目的:回顾关于BRCA2蛋白结构与DNA损伤修复的研究,明确BRCA2蛋白在双链DNA损伤修复中的作用机制。研究选择:选择关于BRCA2蛋白及DNA损伤修复的文献。数据来源:进行全面的检索,检索手段包括电子检索、手工检索及个人通信等。数据提取:对检索到的BRCA2蛋白及DNA损伤修复的相关研究文章信息进行综述。主要观察指标:BRCA2蛋白的结构及其在DNA损伤修复中的作用模型与机制。结果:BRCA2蛋白在肿瘤抑制过程中行使重要功能,其C末端DBD中的DNA结合活性与BRC模体中的RAD51蛋白结合活性在双链DNA损伤修复的同源重组过程中起着直接作用。结论:BRCA2蛋白在双链DNA损伤修复中发挥重要作用,但其具体过程与整个信号通路仍需进一步研究证实,其有望开发为治疗与DNA损伤相关疾病的新靶点,为其预防康复提供理论依据。
BACKGROUND: Breast cancer susceptibility protein 2 (BRCA2) is a tumor suppressor encoded by breast cancer susceptibility gene 2 and plays an important role in maintaining the biological response of chromosome stable and DNA damage in mammalian cells. Many studies have shown that: BRCA2 protein and hereditary breast cancer, ovarian cancer and the incidence of Fanconi’s anemia is closely related to the suspected double-strand DNA damage repair, but the specific role of the model and mechanism remains unclear. Objective: To review the research on BRCA2 protein structure and DNA damage repair, and clarify the mechanism of BRCA2 protein in DNA damage repair. Study choice: Select literature on BRCA2 protein and DNA damage repair. Data sources: To conduct a comprehensive search, including electronic search, manual search and personal communication. DATA EXTRACTION: The related research articles of the retrieved BRCA2 protein and DNA damage repair are summarized. MAIN OUTCOME MEASURES: The structure of BRCA2 protein and its role in DNA damage repair models and mechanisms. Results: The BRCA2 protein plays an important role in tumor suppression. The DNA binding activity of the BRCA2 protein in the C terminus DBD and the RAD51 protein binding activity in the BRC motif play a direct role in the homologous recombination of double-stranded DNA damage repair. CONCLUSION: BRCA2 protein plays an important role in the repair of double-stranded DNA. However, its specific process and the entire signaling pathway still need to be further studied. It is expected to be developed as a new target for the treatment of DNA damage-related diseases and provide a theoretical basis for its prevention. in accordance with.