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【目的】碳、氮代谢是竹子节间伸长的重要生理活动,研究节间短缩的圣音竹及节长正常的回复突变体两者在碳氮代谢上的差异,以期揭示影响竹材利用率的主要性状——节间长度在代谢层面的调控机制。【方法】以圣音竹及其回复突变体节间快速伸长时期的竹笋为材料,对其笋体和笋箨不同部位的有关碳氮代谢主要物质及相关酶活性进行测定。【结果】在笋体的部位,圣音竹的蔗糖和果糖含量、以及酸性蔗糖转化酶活性显著低于其回复突变体,中性蔗糖转化酶活性则显著高于后者,而葡萄糖含量在两者间差别不大;圣音竹的蛋白质含量、谷氨酸脱氢酶活性显著低于其回复突变体,铵态氮含量显著大于后者,硝态氮含量在两者间相差不大。在箨的部位,多数指标差异不显著。【结论】从碳氮代谢的角度看,圣音竹笋体中蔗糖浓度降低和氮代谢异常可能是导致其节间短缩的原因之一。
【Objective】 Metabolism of carbon and nitrogen is an important physiological activity of internode elongation in bamboo. The difference of carbon and nitrogen metabolism in both internode shortening Shengyinzhu and the normal length of node elongation mutant was studied in order to reveal the influence of utilization of bamboo The rate of the main traits - internode length in the metabolic regulation mechanism. 【Method】 The main carbon and nitrogen metabolism related substances and their related enzymes in different parts of their shoots and bamboo shoots were determined by taking the bamboo shoots during the fast internode elongation of Shengyin bamboo and its response mutant. 【Result】 The content of sucrose and fructose, the content of sucrose and fructose, and the activity of sucrose invertase in Shengyin bamboo were significantly lower than those in the recovery mutants. The activity of neutral sucrose invertase was significantly higher than that of the latter, The difference was not significant between the two groups. The protein content and glutamate dehydrogenase activity of Shengyin bamboo were significantly lower than those of the reversion mutant, and the content of ammonium nitrogen was significantly greater than that of the latter, while the content of nitrate nitrogen was not different between the two. In 箨 parts, most of the indicators did not differ significantly. 【Conclusion】 From the perspective of carbon and nitrogen metabolism, the decrease of sucrose concentration and abnormal nitrogen metabolism may be one of the reasons leading to internode shortening.