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目的探讨磷脂酰肌醇3-激酶(PI3K)在卵巢上皮性癌组织中的表达及其与临床病理特征关系。方法采用免疫组织化学SP法检测25例正常卵巢组织、20例良性上皮性卵巢肿瘤和46例卵巢上皮性癌原发灶中的P110α、p85α蛋白表达。结果卵巢上皮性癌中P110α、p85α蛋白阳性表达率显著高于良性和正常卵巢上皮组织(P<0.05)。而P110α、p85α在良性、正常卵巢上皮组织中的阳性表达率差异无统计学意义(P>0.05)。P110α、p85α蛋白表达强度与肿瘤病理类型、术中腹水量、术后残余灶无显著相关性(P>0.05)。P110α、p85α蛋白表达强度与肿瘤病理分期、手术分期和是否伴有淋巴结转移呈显著相关(P<0.05)。P110α、p85α蛋白表达强度在肿瘤低分化组织明显高于高中分化组织;P110α、p85α蛋白表达强度在手术分期Ⅲ和Ⅳ期组明显高于Ⅰ和Ⅱ期组;P110α、p85α蛋白表达强度在有淋巴转移组明显高于无淋巴转移组。46例卵巢癌组织中,P110α、p85α蛋白表达强度在有化疗耐药组织中表达明显高于化疗敏感型组织(P<0.05)。结论在卵巢上皮性癌中PI3K/AKT信号通路中的P110α、p85α存在异常表达,可能与卵巢上皮性癌的发生、发展有关。
Objective To investigate the expression of phosphatidylinositol 3 - kinase (PI3K) in epithelial ovarian cancer and its relationship with clinicopathological features. Methods The expressions of P110α, p85α in 25 cases of normal ovarian tissue, 20 cases of benign epithelial ovarian tumor and 46 cases of primary epithelial ovarian cancer were detected by immunohistochemical SP method. Results The positive rates of P110α and p85α protein in epithelial ovarian cancer were significantly higher than those in benign and normal ovarian epithelial tissues (P <0.05). However, the positive rates of P110α and p85α in benign and normal epithelial ovarian tissues were not significantly different (P> 0.05). The expression of P110α and p85α protein had no significant correlation with tumor pathological type, intraoperative ascites volume and postoperative residual tumor (P> 0.05). The expressions of P110α and p85α protein were significantly correlated with tumor stage, surgical stage and lymph node metastasis (P <0.05). The expression of P110α and p85α protein in tumor tissue was significantly higher than that in the high differentiated tissue. The expression of P110α and p85α protein in stage Ⅲ and Ⅳ was significantly higher than those in stage Ⅰ and Ⅱ. The expression of P110α and p85α in the tissue with lymph node Metastasis group was significantly higher than without lymphatic metastasis group. In 46 cases of ovarian cancer tissues, the expression of P110α, p85α protein in chemotherapy-resistant tissues was significantly higher than that in chemotherapy-sensitive tissues (P <0.05). Conclusion The abnormal expression of P110α and p85α in PI3K / AKT signaling pathway in epithelial ovarian cancer may be related to the occurrence and development of epithelial ovarian cancer.