目的　确定耕牛是否为七日热型、澳洲型钩端螺旋体病的传染源。方法　自钩端螺旋体病患者血液及耕牛尿中分离出的 12株七日热型、澳洲型钩端螺旋体野生株染色体DNA用限制性内切酶EcoRⅠ酶切后 ,进行染色体DNA限制性内切酶图谱及 16S +2 3SrRNA基因限制性内切酶片段长度多态性分析。结果　自钩端螺旋体病患者血液及耕牛尿中分离的同一血清型的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型相同。血清型不同的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型则不相同。结论　耕牛是我国七日热型及澳洲型钩端螺旋体病的传染源 ,这对控制我国七日热型及澳洲型钩端螺旋体病的流行有着非常重要的现实意义 Objective To determine if cattle are hot on the 7th and source of Australian Leptospirosis. Methods Chromosomal DNA of 12 seven-day-heat-type, Leptospira interrogans strains of Leptospiractum isolated from the blood of leptospirosis patients and urine from cattle were digested with restriction endonuclease EcoRⅠ and subjected to restriction endonuclease Enzymatic pattern and 16S +2 3S rRNA gene restriction fragment length polymorphism analysis. Results The chromosomal DNA zymogram and ribonucleic acid of the same serotype of Leptospira isolates isolated from the blood of leptospirosis patients and the urine of cattle were the same. Chromosomal DNA zymogram and ribonucleic acid type of wild strains of different serotypes of Leptospira were different. Conclusions Cattle is the source of infection of seven-day fever and Australian-type Leptospirosis in our country, which has very important practical significance to control the epidemic of seven-day fever-type and Australian-type Leptospirosis