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胰岛素抵抗是导致Ⅱ型糖尿病发生的主要机制之一,它会导致高血糖,最终引发糖尿病发病。目前胰岛素抵抗的细胞模型多样,但是以单一因素诱导造模为主。利用高糖联合高脂诱导HepG2细胞模型,更符合人体发病时高血糖高血脂的状态。实验采用25mmol/L葡萄糖联合不同浓度的油酸软脂酸(摩尔浓度比2:1),于36h测定细胞的糖吸收、糖原含量以及甘油三酯含量,以确定合理的造模浓度。最终用0.5mmol/L的游离脂肪酸联合诱导液建立了胰岛素抵抗HepG2细胞模型,并利用此模型对4种成分改善胰岛素抵抗进行了评价。结果发现,葛根素、甜菊苷、熊果酸和表儿茶素均能有效地改善HepG2细胞模型的胰岛素抵抗,相比较甜菊苷和表儿茶素的效果较好。
Insulin resistance is one of the main mechanisms leading to type 2 diabetes, which leads to hyperglycemia and ultimately to the onset of diabetes. At present, the cell models of insulin resistance are diverse, but predominately induced by a single factor. HepG2 cell model induced by high glucose combined with high fat, more in line with human disease hyperglycemia hyperlipidemia state. Experiments using 25mmol / L glucose combined with different concentrations of oleic palmitate (molar ratio of 2: 1) at 36h measured cell sugar absorption, glycogen content and triglyceride content to determine the reasonable model concentration. Finally, 0.5 mmol / L free fatty acid was used as the inducer to establish a HepG2 cell model of insulin resistance. The model was used to evaluate the four components to improve insulin resistance. The results showed that puerarin, stevioside, ursolic acid and epicatechin all can effectively improve the insulin resistance of HepG2 cell model, which is better than stevioside and epicatechin.