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目的探讨热休克蛋白(HSP)70-1+190(G/C)、HSP70-2+1267(A/G)位点基因多态性与煤尘接触工人早期肺部改变的相关性。方法采用病例-对照研究,随机选取河南省某煤矿的工人210例,其中86例胸片结果判定为“观察对象”的为病例组,124例胸片结果判定为正常的为对照组。应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测HSP70-1+190(G/C)和HSP70-2+1267(A/G)的单核苷酸多态性。结果观察对象与同一煤矿胸片结果显示正常的工人HSP70-1+190(G/C)位点的3种基因型GG、GC和CC及G和C等位基因频率在病例组和对照组之间的分布,差异均有统计学意义(2=13.93,P=0.00;2=8.24,P=0.00),CC基因型个体发生早期肺部改变的风险是GG基因型个体的2.42倍(OR=2.42,95%CI:1.20～4.89)。HSP70-2+1267(A/G)位点的基因型及等位基因的分布在2组之间差异均无统计学意义(P>0.05)。结论 HSP70-1+190(G/C)位点多态性可能与煤尘接触工人早期肺部改变相关,携带CC基因型煤尘接触工人更易发生早期肺部改变。
Objective To investigate the relationship between polymorphisms of heat shock protein (HSP) 70-1 + 190 (G / C) and HSP70-2 + 1267 (A / G) loci and early pulmonary changes in coal dust exposed workers. Methods A case-control study was conducted in which 210 workers were randomly selected from a coal mine in Henan Province. Among them, 86 cases of chest radiograph were judged as “observation object” and 124 cases of chest radiograph were determined as normal. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect single nucleotide polymorphisms . Results The frequencies of GG, GC, CC, G and C alleles of the three genotypes in HSP70-1 + 190 (G / C) sites in the observation subjects and the same mine chest radiographs were normal in case group and control group (2 = 13.93, P = 0.00; 2 = 8.24, P = 0.00). The risk of early pulmonary changes in individuals with CC genotype was 2.42 times higher than that in individuals with GG genotype (OR = 2.42, 95% CI: 1.20 ~ 4.89). The genotype and allele distribution of HSP70-2 + 1267 (A / G) loci showed no significant difference between the two groups (P> 0.05). Conclusion Polymorphism of HSP70-1 + 190 (G / C) loci may be associated with early pulmonary changes in workers exposed to coal dust. Early lung changes may be more likely to occur in coal dust exposed workers with CC genotypes.