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目的探讨柯萨奇B组病毒(CVB)感染与哮喘发作的关系。方法观察急性发作期哮喘患儿102例、上呼吸道感染182例、非感染性疾病患儿71例、健康儿童30例。采用ELISA法测定静脉血CVB抗原(CVB—Ag)和IgM抗体(CVB—IgM)、免疫组化法测定T细胞亚群和[3H)—TdR标记法测定NK细胞活性。结果①哮喘患儿CVB感染率为57.8%(59/102);②哮喘患儿CD8显著升高;CD3、CD4/CD8及NK细胞活性均明显下降(P<0.01);而CVB阳性哮喘患儿NK细胞活性又低于非CVB阳性哮喘儿(P<0.05),CD8明显高于非CVB阳性哮喘儿(P<0.01)。结论CVB感染与哮喘发作有密切关系。
Objective To investigate the relationship between coxsackie virus (CVB) infection and asthma attack. Methods 102 cases of asthma, 182 cases of upper respiratory tract infection, 71 cases of non-infectious disease and 30 healthy children were observed during the acute episode. CVB-Ag and CVB-IgM were detected by ELISA. T cell subsets and [3H] -TdR labeling were used to determine NK cell activity. Results ① The CVB infection rate in asthmatic children was 57.8% (59/102). ② The levels of CD8, CD3, CD4 / CD8 and NK cells in asthmatic children were significantly increased (P <0.01), while those in CVB-positive asthmatic children NK cell activity was lower than non-CVB positive asthmatic children (P <0.05), CD8 was significantly higher than non-CVB positive asthmatic children (P <0.01). Conclusion CVB infection is closely related to asthma attack.