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目的 :探讨Fc受体γ链基因 3′端非翻译区 (3′ -UTR) 380 4位点T/G多态现象在中国南方汉族人群中的分布及与系统性红斑狼疮 (SLE)的关系。方法 :采用PCR -RFLP方法分析Fc受体γ链基因 3′ -UTR 380 4位点T/G单碱基替换的多态现象。结果 :在 16 8例SLE患者和 12 0例正常人中只发现 1例SLE患者存在该位点的T/G杂合现象 ,该杂合现象经基因测序证实 ,其余均位TT型。结论 :Fc受体γ链基因 3′ -UTR 380 4位点T/G单碱基替换的多态性现象在中国南方汉族人群中少见 ,该位点的基因多态现象与SLE发病无关。
OBJECTIVE: To investigate the distribution of T / G polymorphism at the 3’-UTR of the 3 ’-UTR gene of Fc receptor γ chain in Han population of southern China and its relationship with systemic lupus erythematosus (SLE) . Methods: PCR-RFLP method was used to analyze the polymorphism of T / G single nucleotide substitution at the 3’-UTR 380 4 locus of Fc receptor γ chain gene. Results: T / G heterozygosity was found in only one patient with SLE in 168 SLE patients and 12 0 normal subjects. The heterozygosity was confirmed by sequencing and all other TT genotypes. CONCLUSION: Polymorphism of T / G single nucleotide substitution at the 3 ’-UTR 380 site of Fc receptor γ chain gene is rare in southern Chinese Han population. The polymorphism of this gene has nothing to do with the pathogenesis of SLE.