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目的 探讨GST πmRNA检测在食管癌发生发展中的作用。方法 在来自食管癌高发区的 2 2例配对的食管癌组织和非食管癌组织中 ,应用AP PCR技术检测差异基因片段 ,应用RT PCR检测GST πmRNA的表达。结果 6例癌组织中存在差异随机扩增片段 ,而相应的非癌组织中则缺如。其中 5T差异基因片段为 1.0kb ,经克隆、测序和序列同源性分析 ,基因文库内无同源序列。GST πmR NA在非癌组织和癌组织中的表达阳性率分别为 18.2 %(4 / 2 2 )和 5 4.5 %(12 / 2 2 )。结论 5T差异基因片段是否为一个新候选基因或癌基因标志物 ,有待进一步筛查。GST πmRNA表达在食管组织癌变过程中明显增强。
Objective To investigate the role of GST π mRNA in esophageal carcinogenesis. Methods Two pairs of matched esophageal cancer tissues and non-esophageal cancer tissues from esophageal cancer-prone areas were detected by AP PCR. The expression of GST πmRNA was detected by RT-PCR. Results There were differences in 6 cases of cancer tissue randomly amplified fragments, while the corresponding non-cancerous tissue is absent. Among them, the 5T differential gene fragment was 1.0kb. There was no homologous sequence in the gene library after cloning, sequencing and sequence homology analysis. The positive rates of GST πmR NA in non-cancerous and cancerous tissues were 18.2% (4/2 2) and 4.5% (12/2 2), respectively. Conclusion Whether the 5T differential gene fragment is a new candidate gene or oncogene marker remains to be further screened. GST πmRNA expression in esophageal carcinogenesis was significantly enhanced.