目的:比较2种胚胎培养模式对胚胎质量和妊娠结局的影响。方法:收集首次行IVF-ET治疗、年龄<38岁、采用长方案促排卵、常规IVF授精、正常受精卵数>3枚且第3日有新鲜胚胎移植的518个周期的临床资料,根据正常受精卵采用的培养模式分成单胚胎培养组(n=254)和胚胎群组培养组(n=264)。分析患者的基本资料、胚胎发育情况以及妊娠结局。结果:患者的年龄、不孕年限、基础卵泡刺激素(FSH)、Gn用量、h CG注射日内膜厚度以及各种不孕因素的比例等参数组间差异均无统计学意义(P>0.05);平均获卵数、成熟率、2PN率等指标组间也均无统计学差异(P>0.05),但胚胎群组培养组的卵裂率、第3日优质胚胎率、剩余胚胎可利用囊胚形成率均明显高于单胚胎培养组,差异有统计学意义(P<0.05);胚胎群组培养组的种植率和临床妊娠率高于单胚胎培养组,但差异无统计学意义(P>0.05)。结论:采用胚胎群组培养模式可以得到更多的优质胚胎和更高的囊胚形成率,推荐可用于日常IVF治疗周期中的胚胎培养。 Objective: To compare the effects of two embryo culture modes on embryo quality and pregnancy outcome. Methods: The clinical data of 518 cycles of first embryo IVF-ET, aged <38 years, ovulation induction with long protocol, routine IVF insemination, normal fertilized eggs> 3, and fresh embryo transfer on the third day were collected. The culture patterns of fertilized eggs were divided into single embryo culture group (n = 254) and embryo group culture group (n = 264). Analysis of the patient’s basic information, embryonic development and pregnancy outcomes. Results: There was no significant difference in parameters such as patient’s age, duration of infertility, basal follicle stimulating hormone (FSH), Gn dosage, intima-media thickness on h CG injection and various infertility factors (P> 0.05 ). The mean number of oocytes retrieved, maturity rate and 2PN rate were also not significantly different (P> 0.05). However, the rate of cleavage in embryo group, the rate of high-quality embryo on day 3, the available embryos Blastocyst formation rate was significantly higher than single embryo culture group, the difference was statistically significant (P <0.05); embryo group culture group implantation rate and clinical pregnancy rate was higher than single embryo culture group, but the difference was not statistically significant P> 0.05). CONCLUSIONS: Higher quality embryos and higher blastocyst formation rates can be obtained using embryo cohort culture and are recommended for embryo culture in routine IVF cycles.