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利用气相色谱研究人尿中甾体激素的代谢产物已有不少报导,一般利用硅烷化试剂与甾体分子中所含羟基反应,制备成三甲基硅醚(TMS)衍生物后再进行气相色谱分析。在室温下,除了17-α的叔羟基和11-β立体位阻较大的羟基外,其余位置的羟基均可顺利反应,重现性好。对于同时含有酮基和羟基的分子可先使酮基反应生成甲肟(MO)衍生物,然后再进行三甲基硅烷化。 Stillwell等曾应用色谱-质谱联用技术研究了甾体避孕药的代谢产物。我们在研究长效口服避孕药-环戊醚乙炔雌二醇(CEE)对尿中中性激素代谢产物影响的工作中,着重试验了雄激素和孕激素代
Gas chromatography has been used to study the metabolites of steroid hormones in human urine. There are many reports on the metabolites of steroid hormones in human urine. Gas chromatography is generally used to react trimethylsilyl ether (TMS) with the hydroxyl groups contained in steroid molecules. spectrum analysis. At room temperature, in addition to the 17-α tertiary hydroxyl and 11-β steric hindrance of the hydroxyl group, the remaining hydroxyl groups can be successfully reacted with good reproducibility. For molecules containing both keto and hydroxyl groups, the keto group can be first reacted to form the methyloxime (MO) derivative, followed by trimethylsilylation. Stillwell et al. Have used chromatographic-mass spectrometry techniques to study the metabolites of steroidal contraceptives. In our study of the effects of long-acting oral contraceptives, cyclohexylacetate ethinyl estradiol (CEE) on urinary neutral hormone metabolites, we focused on testing for androgen and progestin