人红细胞通过其膜上的葡萄糖载体蛋白来转运葡萄糖,此载体只转运D型葡萄糖,是一被动转运过程.由于人红细胞膜上葡萄糖载体含量较多,活性也较高,因此一般都将它作为糖转运模型来研究.但迄今为止,对其结构、作用机制及活性调节等都不甚明了.尤其对细胞内二价阳离子,如钙离子等,对它的调节更少见报道.对正常的红细胞,其质膜内外存在着一个大约1000倍的钙离子梯差,如果细胞内钙离子浓度升高,使此梯差变小甚至消失,就会影响膜蛋白的活性,导致一些异常的生理反应.我们曾报道,胞内钙离子浓度升高会明显地抑制完整红细胞葡萄糖载体活性,其半抑制浓度约为250μmol/L,但完整红细胞内有许多胞质 Human erythrocytes transport glucose through the glucose carrier protein on its membrane, which only transports D-glucose, which is a passive transport process. Because human erythrocyte membrane contains more glucose carrier and higher activity, it is generally regarded as Sugar transport model to study.But so far, its structure, mechanism of action and activity regulation are not clear.Especially for intracellular divalent cations, such as calcium, its regulation is seldom reported.For normal red blood cells , There is an about 1000-fold increase in calcium ion inside and outside the plasma membrane. If the concentration of intracellular calcium is increased, this step difference will be smaller or even disappear, which will affect the activity of membrane protein and lead to some abnormal physiological responses. We have reported that elevated intracellular calcium concentration significantly inhibits intact erythrocyte glucose vector activity with a half-inhibitory concentration of about 250 μmol / L, but there are many cytoplasm within intact erythrocytes