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单片微机化微弱发光测量仪由中科院生物物理所和北京科龙生物医学技术开发公司研制成功.该仪器适用于肿瘤研究中生物医学样品的微弱发光的测量.测量样品的重复性优于0.8%,稳定性优于0.5%,线性相关系数达0.99997,不仅可以进行样品的发光强度测量,还可以进行动力学曲线的测量以及样品发射光谱的测量.光谱在400nm—750nm范围内.测量方式可选择手动、半自动和自动计时三种.测量结果表明,荷瘤裸鼠血液微弱发光与对照比较,在99%置信水平上有明显差异.荷瘤鼠各脏器发光强度的相对分布有较大改变;脑和肝发光强度所占比重有明显增高.实验鼠整体发光在荷瘤后14—34天内显著增强.
The monolithic microcomputerized weak luminescence measuring instrument was successfully developed by the Institute of Biophysics, Chinese Academy of Sciences and Beijing Kelong Biomedical Technology Development Co., Ltd. This instrument is suitable for the measurement of weak luminescence of biomedical samples in tumor research. The reproducibility of the measured sample is better than 0.8%. The stability is better than 0.5%, and the linear correlation coefficient is 0.99997. It can not only measure the luminous intensity of the sample, but also can measure the kinetic curve and measure the emission spectrum of the sample. The spectrum is in the range of 400nm-750nm. The measuring method can be selected. Manual, semi-automatic, and automatic timekeeping are three types. The measurement results show that compared with the control, the blood faint luminescence of the tumor-bearing nude mice is significantly different at the 99% confidence level. The relative distribution of the luminescence intensity of the organs of the tumor-bearing mice is greatly changed. The proportion of brain and liver luminescence intensity was significantly increased. The whole luminescence of mice in the experiment significantly increased within 14 to 34 days after tumor implantation.