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目的:研究骨形成蛋白受体(BMP-R)在大鼠骨髓间充质细胞(BMSCs)成骨中的作用机制。方法:分离大鼠BMSCs,将原代细胞分为4组,分别应用普通培养基(CM)、成骨诱导培养基(OM)、骨形成蛋白-2(BMP-2)和OM+BMP-2诱导培养基培养。应用碱性磷酸酶(ALPase)活性和钙结节染色(Von Kossa法)检测成骨分化程度,RT-PCR检测骨形成蛋白受体(BMP-R)的表达情况。采用SPSS16.0软件包对数据进行单因素方差分析。结果:OM可诱导BMSCs成骨分化,表现出高碱性磷酸酶活性和基质矿化能力。BMP-2可提高OM诱导BMSCs成骨分化的能力;OM在早期即可诱导BMP-RⅠA和BMP-RⅡ的表达,而BMP-2诱导BMP-R较迟。结论:BMP-2可提高OM诱导BM-SCs分化成骨的能力,BMP-R在BMSCs分化成骨中起重要作用。
OBJECTIVE: To study the mechanism of bone morphogenetic protein receptor (BMP-R) in the osteogenesis of rat bone marrow mesenchymal cells (BMSCs). Methods: Primary BMSCs were isolated and divided into 4 groups: primary culture medium (CM), osteogenic induction medium (OM), bone morphogenetic protein-2 (BMP-2) and OM + BMP-2 Induction culture medium. The degree of osteogenic differentiation was detected by alkaline phosphatase (ALPase) activity assay and calcium nodule staining (Von Kossa method). The expression of BMP-R was detected by RT-PCR. Data were analyzed by one-way ANOVA using SPSS16.0 software package. Results: OM could induce osteogenic differentiation of BMSCs and showed high alkaline phosphatase activity and matrix mineralization. BMP-2 can enhance the ability of OM to induce osteogenic differentiation of BMSCs; OM can induce the expression of BMP-RⅠA and BMP-RⅡ at an early stage, whereas BMP-2 can induce BMP-R later. Conclusion: BMP-2 can enhance the ability of OM to induce osteogenic differentiation of BM-SCs. BMP-R plays an important role in the differentiation of BMSCs into osteoid.