【摘 要】
：
细胞内抗体是指能够在细胞内特定区域特异性中和靶蛋白功能的抗体。内抗体技术是一种翻译后水平上抑制靶蛋白质功能的技术,已经成为一种功能基因组学研究的重要工具。本文综
【机 构】
：
军事医学科学院放射医学研究所基因组学与蛋白质组学研究室,
论文部分内容阅读
细胞内抗体是指能够在细胞内特定区域特异性中和靶蛋白功能的抗体。内抗体技术是一种翻译后水平上抑制靶蛋白质功能的技术,已经成为一种功能基因组学研究的重要工具。本文综述了细胞内抗体转运和作用机制,分析了细胞内抗体技术和其他靶向敲除技术的优、缺点,以及细胞内抗体技术存在的问题及解决途径。
An intracellular antibody refers to an antibody that specifically neutralizes the function of a target protein in a specific region of the cell. Internal antibody technology is a post-translational technique that inhibits the function of target proteins and has become an important tool for functional genomics research. This review summarizes the mechanism of intracellular antibody transport, analyzes the advantages and disadvantages of intracellular antibody and other targeted knockout techniques, and the problems existing in intracellular antibody technology and their solutions.
其他文献
A better understanding of the mechanisms of water uptake by plant roots should be vital for improving drought resistance and water use efficiency (WUE). In the
AIM: To study the clinicopathological significance of p53 and mdm2 protein expression in human pancreatic cancer. METHODS: To investigate the expression of p53
The histopathological features and the associated clinical findings of ulcerative colitis (UC) are due to persistent inflammatory response in the colon mucosa.
采用热扩散式探针(TDP)对湿地松(Pinus elliottii)树干液流密度及其周围环境因子进行长期同步测定,同时辅以树干解析、枝条解析等树木生长量调查方法,以期探索不同尺度上湿地
以环洞庭湖区平湖旱地为研究区域,采用田间原位监测方法,通过设置常规施肥(N375kg/hm2和P67.5kg/hm2)、减量10%、20%、30%和不施肥对照处理,研究了不同施肥条件对棉花植株发
以直链烷基苯(linear alkyl benzenes,LABs)为人文活动示踪物,研究其在黄河三角洲潮间带环境中的分布和降解特征。沉积物样品采集于2007年9月、2008年4月和7月,结果表明,调查
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7
In this study, by analysis of genome structures of E. coli, the relationships Between the genomic types of E. coli and the associated diseases were investigated
神经元发育过程中轴突和树突的分化和形成是神经元极化建立的标志,也是建立神经信号转导的基础.近年来,神经元极化的分子机制有了重大突破,发现神经元细胞骨架微丝和微管的结