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目的观察芹菜素(Apigenin,AP)对雄性大鼠肝、脑、睾丸、肾和血清脂质过氧化的影响,并探究其作用机制。方法 48只SD雄性大鼠随机分为对照组(生理盐水10 ml/kg)、低剂量组(AP 234 mg/kg)、中剂量组(AP 468 mg/kg)和高剂量组(AP936 mg/kg),灌胃35 d后测定血清和肝、脑、睾丸、肾的总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)、谷胱甘肽(GSH)含量。结果肝和血清234、468和936 mg/kg剂量组及肾468和936 mg/kg剂量组T-AOC低于对照组(P<0.05),睾丸234和468 mg/kg剂量组T-AOC高于对照组、936 mg/kg剂量组低于对照组(P<0.05),脑468和936 mg/kg剂量组T-AOC高于对照组(P<0.05);肝234 mg/kg剂量组MDA含量高于对照组,468和936 mg/kg剂量组低于对照组(P<0.05),睾丸234 mg/kg剂量组MDA含量低于对照组(P<0.05),肾468 mg/kg剂量组MDA含量低于对照组、936 mg/kg剂量组高于对照组(P<0.05);脑468 mg/kg剂量组和睾丸234、468和936 mg/kg剂量组GSH含量低于对照组(P<0.05);肝芹菜素组SOD、CAT、GSH-Px活力低于对照组,睾丸芹菜素组SOD、GSH-Px活力低于对照组,脑芹菜素组SOD、GSH-Px活力高于对照组(P<0.05),肾和血清抗氧化酶变化复杂。结论芹菜素能影响大鼠肝、脑、睾丸、肾、血清的抗氧化系统,但不一致:在本实验条件下,芹菜素在脑组织表现为抗氧化作用,在睾丸组织较低剂量下表现为抗氧化作用、较高剂量下表现为促氧化作用,在肝、血清、肾表现为促氧化作用;芹菜素对大鼠脏器脂质过氧化和总抗氧化能力的影响不一致。
Objective To observe the effect of apigenin (AP) on lipid peroxidation in liver, brain, testis, kidney and serum of male rats and explore its mechanism. Methods 48 SD male rats were randomly divided into control group (10 ml / kg normal saline, AP 234 mg / kg), AP 468 mg / kg and AP936 mg / kg). The total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione Peroxidase (GSH-Px) activity and malondialdehyde (MDA), glutathione (GSH) content. Results T-AOC in serum of 234, 468 and 936 mg / kg and renal 468 and 936 mg / kg of liver and serum were lower than those of control group (P <0.05). T-AOC of testis at 234 and 468 mg / In the control group, the T-AOC in the 936 mg / kg dose group was lower than that in the control group (P <0.05), and the T-AOC values in the 468 and 936 mg / (P <0.05). The content of MDA in the testis 234 mg / kg group was lower than that in the control group (P <0.05), and the dose of 468 mg / kg dose group was lower than that of the control group (P <0.05). The GSH levels in the 468 mg / kg and 234,468 and 936 mg / kg groups were lower than those in the control group (P <0.05) <0.05). The activities of SOD, GSH-Px, SOD and GSH-Px in the apigenin group were lower than those in the control group, and the activities of SOD and GSH-Px in the apigenin group were lower than those in the control group (P <0.05), and the changes of antioxidant enzymes in kidney and serum were complicated. Conclusion Apigenin can affect the antioxidative system of liver, brain, testis, kidney and serum in rats, but it is inconsistent. Under the experimental conditions, apigenin shows antioxidative effect in brain tissue, Antioxidant effects showed the pro-oxidant effect at higher dose, and pro-oxidant activity in liver, serum and kidney. The effects of apigenin on lipid peroxidation and total anti-oxidative capacity in rats were inconsistent.