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目的研究食蟹猴老化过程中运动行为和脑内纹状体多巴胺系统功能变化及两者之间的相关关系。方法选取4岁、10岁和15岁3个年龄组的健康食蟹猴共29只,利用计算机化的网络摄像头视频检测系统和行为分析软件连续采集和分析每个动物8h随意运动活动总量,各年龄组分别选取4只动物用多巴胺转运体(DAT)配体99mTc-TRODAT-1结合单光子发射体层摄影术(SPECT)显像观察脑内纹状体多巴胺转运体放射性摄取率的变化。结果在4岁、10岁和15岁年龄组,8h随意运动活动总量(×106)分别为5·00±1·93,3·28±1·02,2·79±0·67,在10岁和15岁较之4随年龄组分别降低了34·50%和55·71%(P<0·05,P<0·01),但此两个年龄组运动活动总量无显著差异(P>0·05);纹状体99mTc-TRODAT-1放射性摄取率分别为2·98±0·08,2·56±0·12和2·27±0·35,10岁和15岁较之4随年龄组分别降低了14·00%和25·60%,但仅4岁与15岁年龄组存在显著相关关系(P<0·01)。二者均随着年龄的增长呈逐渐减低的趋势,直线回归分析显示两者分别与年龄呈负相关关系(r=-0·57,P=0·001;r=-0·86,P<0·01)。8h随意运动活动总量与纹状体99mTc-TRODAT-1放射性摄取率呈显著的正相关关系(r=0·70,P<0·05)。结论正常食蟹猴老化过程中,脑内多巴胺神经系统功能的减退伴随着运动行为的减少,两者之间的相关关系进一步佐证了运动功能的减退可能是由于纹状体内多巴胺神经元功能减退所致。
Objective To study the changes of motor function and the function of striatum dopaminergic system in cynomolgus monkeys during aging and the relationship between them. Methods A total of 29 healthy cynomolgus monkeys (aged 4, 10 and 15 years old) were selected. The computerized webcam video detection system and behavior analysis software were used to continuously collect and analyze the total amount of voluntary exercise activities of each animal for 8 hours. In each age group, four animals were selected to observe the changes of radioactive uptake of dopamine transporter in the striatum of the brain by 99mTc-TRODAT-1 dopamine transporter (DAT) combined with single photon emission tomography (SPECT) imaging. Results In 4 years, 10 years and 15 years of age group, the total amount of voluntary exercise at 8 hours (× 106) was respectively 5.00 ± 1.93, 3.28 ± 1.22, 2.79 ± 0.67, Compared with the age group of 10 and 15, the average age of 10 and 15 decreased by 34.5% and 55.71% respectively (P <0.05, P <0.01), but there was no significant difference between the two age groups (P> 0.05). The radioactive uptake rates of 99mTc-TRODAT-1 in striatum were 2.98 ± 0.08, 2.56 ± 0.12 and 2.27 ± 0.35, respectively. Compared with the four age groups, respectively, decreased by 14.00% and 25.60%, but only 4 years old and 15 years old age group there is a significant correlation (P <0.01). Both of them showed a trend of decreasing gradually with age. The linear regression analysis showed that the two were negatively correlated with age (r = -0.57, P = 0.001, r = -0.86, P < 0 · 01). There was a significant positive correlation between the amount of voluntary exercise activities and radioactive uptake of 99mTc-TRODAT-1 in striatum at 8h (r = 0.70, P <0.05). Conclusions During normal aging of crab, the decrease of brain function of dopaminergic neurons accompanied with the decrease of motor behavior, and the correlation between them further corroborates that the decrease of motor function may be due to the decrease of dopamine neurons in the striatum To