目的:探讨冻融胚胎移植(FET)周期中影响胚胎种植潜能的相关因素。方法:回顾性分析165例进行冻融胚胎并实施单胚胎移植(SET)的患者,按患者年龄、冷冻前胚胎质量、冷冻方式、复苏后卵裂球存活状态、复苏后是否进行辅助孵化分组,分析不同分组各组间的胚胎种植能力差异。结果:年龄≥36岁组的胚胎种植率(17.4%)低于年龄<36岁组(26.1%),但两者比较无统计学差异;冷冻前胚胎质量评分≥20组的胚胎种植潜能优于胚胎质量评分<20组(29.4%vs13.0%,P<0.05);玻璃化快速冷冻法和程序慢速冷冻法对胚胎种植能力的影响无明显差异(25.6%vs24.6%,P>0.05);复苏后卵裂球完整存活的胚胎种植率为29.8%,优于复苏后卵裂球部分损伤者(13.7%)(P<0.05);激光辅助孵化对胚胎种植能力无明显影响(21.4%vs25.2%,P>0.05)。结论:冷冻前胚胎质量及冷冻后胚胎存活状态是影响冻融胚胎种植潜能的重要因素。 Objective: To investigate the factors influencing the potential of embryo implantation in frozen-thawed embryo transfer (FET) cycles. Methods: A retrospective analysis of 165 patients with frozen-thawed embryos and single embryo transfer (SET) was performed. Patients were divided into two groups according to the patient’s age, pre-freezing embryo quality, freezing mode, survival status of the blastomere after resuscitation, Analysis of different groups of embryos in different groups ability to grow. Results: Embryo implantation rate (17.4%) in the group of ≥36 years old was lower than that in the group of <36 years old (26.1%), but there was no significant difference between the two groups There was no significant difference in embryo implantation capacity between embryo quality score <20 (29.4% vs 13.0%, P <0.05). There was no significant difference in embryo implantation capacity between vitrification and slow freezing methods (25.6% vs 24.6%, P> 0.05 ). The embryo implantation rate of the surviving blastomeres was 29.8% after resuscitation, which was better than that of the blastocysts (13.7%) after resuscitation (P <0.05). Laser-assisted hatching had no effect on the ability of embryo implantation (21.4% vs25.2%, P> 0.05). Conclusion: The quality of pre-frozen embryos and the post-frozen embryo survival status are the important factors affecting the potential of frozen-thawed embryos.