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目的 研究藻酸双酯钠 (PSS)对豚鼠心室肌细胞动作电位和L 型钙电流的影响。方法 采用全细胞膜片钳记录技术。结果 PSS明显缩短动作电位时程 (APD) ,5 0、10 0和 2 0 0mg·L-1浓度 ,分别使APD90 缩短 2 4 9% (P <0 0 5 ,n=6 )、37 7%和 4 7 7% (P <0 0 1,n =6 ) ,使APD50 缩短2 1 1% (P <0 0 5 ,n =6 )、4 5 0 %和 6 3 2 % (P <0 0 1,n =6 ) ,呈明显的剂量依赖关系。PSS浓度依赖性减小L 型钙电流 (Ica L) ,5 0、10 0和 2 0 0mg·L-1浓度 ,分别使其峰值降低34 3% (P <0 0 5 ,n =6 )、5 8 4 %和 74 9% (P <0 0 1,n =6 )。随着PSS浓度的增加 ,L 型钙电流的I U曲线逐渐上移 ,但其最大激活电压保持为 0mV不变。结论 PSS明显缩短APD ,抑制Ica L,且呈浓度依赖关系
Objective To study the effects of sodium alginate (PSS) on the action potentials and L-type calcium current of guinea pig ventricular myocytes. Methods Whole-cell patch clamp recording technique was used. Results PSS significantly shortened APD, 50, 100 and 200 mg · L-1, respectively, which reduced APD90 by 24.9% (P <0.05, n = 6) and 37.7% And 47.7% (P <0.01, n = 6), respectively, which shortened APD50 by 21.1% (P <0 05, n = 6), 450% and 6 3 2% 1, n = 6), showing a significant dose-dependent relationship. PSS concentration-dependent decrease of L-type calcium current (Ica L), 50,10 and 200 mg · L-1, respectively, decreased the peak value by 34 3% (P 0 05, n = 6) 5 8 4% and 74 9% (P <0 0 1, n = 6). With the increase of PSS concentration, the I U curve of L-type calcium current gradually moved up, but its maximum activation voltage remained unchanged at 0 mV. Conclusion PSS significantly shortened APD and inhibited Ica L in a concentration-dependent manner