论文部分内容阅读
[目的]本文旨在研究水稻籼粳交杂种优势的分子基础。[方法]利用Affymetrix水稻表达谱芯片分析了强优势水稻籼粳交组合‘申9A/繁31’与母本‘申9A’和父本‘繁31’在幼穗分化初期剑叶组织的基因表达差异,并对差异表达基因进行了基于生物学过程的GO(gene ontology)功能分类和代谢通路分析。[结果]幼穗分化初期杂种与双亲的表型已经产生了明显差异;共有2 258个基因在杂种与亲本之间差异表达(差异倍数≥2或≤0.5),杂种与双亲均显示差异表达的基因有327个,杂种与父本之间的差异表达基因远多于母本。杂种差异表达基因具有多种表达模式,具有正效应基因的增强表达和具有负效应基因的抑制表达对杂种优势均有贡献。差异表达基因主要参与了磷代谢、运输、防卫反应以及碳水化合物代谢等生物学过程,并在碳固定、氨基酸代谢、糖酵解/糖原异生以及磷酸肌醇代谢等多个代谢通路富集。[结论]水稻籼粳交杂种优势存在复杂的分子基础,多种分子机制参与了杂种优势的形成,光合效率和光合产物运输分配效率的提高可能是杂种优势产生的主要原因。
[Objective] The aim of this paper is to study the molecular basis of indica-japonica cross hybrid vigor in rice. [Method] Affymetrix rice cDNA microarray was used to analyze the gene expression of flag leaf tissue in the early stage of spike differentiation between indica and japonica crosses (’Shen 9A / Fan 31’) and female parent ’Shen 9A’ Differences in gene expression and differential gene expression based on biological processes GO (gene ontology) functional classification and metabolic pathways. [Result] The phenotypes of hybrids and parents had a significant difference at the early stage of spike differentiation. A total of 2 258 genes were differentially expressed between the hybrids and their parents (with a multiple of 2 or greater than or equal to 0.5), and both hybrids and parents showed differentially expressed There are 327 genes, and there are far more differentially expressed genes between the hybrid and the male parent than the female parent. Hybrid differential expression of genes with a variety of expression patterns, with positive effect of gene expression and negative expression of genes have inhibitory effects on heterosis contribute. Differentially expressed genes are mainly involved in biological processes such as phosphorus metabolism, transport, defense response and carbohydrate metabolism, and are enriched in a number of metabolic pathways such as carbon fixation, amino acid metabolism, glycolysis / gluconeogenesis and phosphoinositide metabolism . [Conclusion] There existed complicated molecular basis for the hybrid heterosis in indica-japonica hybrid rice. Various molecular mechanisms were involved in the formation of heterosis. The increase of photosynthetic efficiency and photosynthetic transport efficiency could be the main reason for heterosis.