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以辣椒“A11”和黄瓜炭疽病菌(Colletotrichum orbiculare)组成的非寄主互作体系为研究对象,利用组织化学方法研究了辣椒叶片微管骨架解聚后接种黄瓜炭疽病菌,对抗病反应相关的乳突、H2O2和过敏性坏死反应的影响以及黄瓜炭疽病菌在非寄主辣椒上的侵入状况,揭示微管骨架在辣椒-黄瓜炭疽病菌非寄主互作中的作用。结果表明,微管骨架解聚显著抑制了乳突的形成,H2O2的积累也明显减弱,使黄瓜炭疽病菌能成功侵入辣椒细胞,表明微管骨架参与了辣椒的非寄主抗病性反应,微管的聚合在辣椒非寄主抗病性中具有重要的作用。
The non-host interaction system composed of pepper “A11” and Colletotrichum orbiculare was used as the research object. The histochemical method was used to study the pathogenicity of anthracnose inoculated by the microtubule framework of pepper leaves after inoculation. , The effects of H2O2 and anaphylactoid necrosis, and the invasion of anthracnose on non-host chili peppers, revealing the role of microtubule cytoskeleton in non-host interactions of pepper-cucumber anthracnose. The results showed that the depolymerization of microtubules significantly inhibited the formation of papillae and the accumulation of H2O2 was also significantly weakened, so that the cucumber anthracnose could successfully invade the pepper cells, indicating that the microtubule framework was involved in the non-host disease resistance of pepper and microtubules Polymerization plays an important role in pepper non-host disease resistance.