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目的 探讨BRCA1基因蛋白在汉族散发性乳腺癌中的表达及其临床病理意义。方法 采用免疫组化SP法对 5 1例乳腺癌石蜡切片组织进行BRCA1检测 ,并分析它与临床病理指标及c erbB 2表达的关系。 结果 BRCA1蛋白定位于癌细胞核 ,其失表达和低表达率为 49%。BRCA1蛋白表达下调与c erbB 2表达相关 (tr=4 15 5 ,P <0 0 5 ) ,与组织学分级、肿块大小、淋巴结转移数目及患者年龄均无明显相关。 结论 BRCA1蛋白表达的检测可在一定程度上反映基因的改变 ,可作为乳腺癌易感性初筛的手段。
Objective To investigate the expression of BRCA1 protein in Han sporadic breast cancer and its clinicopathological significance. Methods The immunohistochemical SP method was used to detect the BRCA1 expression in 5 1 breast cancer paraffin sections and its relationship with clinicopathological features and c erbB 2 expression. Results The BRCA1 protein localized in the nucleus of cancer cells, and its loss of expression and low expression rate was 49%. The down-regulation of BRCA1 protein expression was correlated with the expression of c-erbB 2 (tr = 4 15 5, P 0 05), but not with histological grade, tumor size, lymph node metastasis and patient’s age. Conclusion The detection of BRCA1 protein expression can reflect the change of gene to some extent, which can be used as a preliminary screening method of breast cancer susceptibility.