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目的 研究人垂体生长激素（GH）腺瘤临床生化特征及与肿瘤Gsp癌基因表达、激素分泌、细胞增殖水平及侵袭性之间的相互关系。方法 垂体GH腺瘤43例,对其中17例分别提取DNA,经多聚酶链反应（PCR）扩增和基因直接序列分析法检测Gsp癌基因的表达;以125I-VolR DNA聚合酶活性测量法,检测43例患者血清DNA聚合酶活性以间接评价肿瘤组织增生活性;用放射免疫法测定患者血清GH分泌水平。结果Gsp癌基因阴性和阳性患者在肿瘤大小、GH水平及侵袭性上均无显著性差异。侵袭性垂体腺瘤DNA聚合酶活性比非侵袭性垂体腺瘤患者高,在统计学上有显著性差异（P<0.05）。侵袭性垂体腺瘤患者的GH水平明显高于非侵袭性垂体腺瘤,且有统计学意义（P<0.05）。结论 侵袭性垂体腺瘤的增殖活性和GH水平明显增高。DNA聚合酶活性和GH水平可作为判断垂体腺瘤侵袭性的有用参考指标。
Objective To study the clinical biochemical characteristics of pituitary growth hormone (GH) adenoma and its relationship with Gsp oncogene expression, hormone secretion, cell proliferation and invasiveness. Methods Forty-three cases of pituitary GH adenoma were included in the study. DNA was extracted from 17 cases, and the expression of Gsp oncogene was detected by polymerase chain reaction (PCR) amplification and direct sequence analysis of genes. The activity was measured by 125I-VolR DNA polymerase activity assay. The activity of serum DNA polymerase in 43 patients was used to indirectly evaluate the proliferative activity of tumor tissue. Serum GH secretion was measured by radioimmunoassay. Results There was no significant difference in tumor size, GH level and invasiveness between Gsp oncogene negative and positive patients. The DNA polymerase activity of invasive pituitary adenomas was higher than that of non-invasive pituitary adenomas. There was a statistically significant difference (P<0.05). The GH level of invasive pituitary adenomas was significantly higher than that of non-invasive pituitary adenomas, with statistical significance (P<0.05). Conclusion The invasive pituitary adenomas have significantly increased proliferative activity and GH levels. DNA polymerase activity and GH levels can be used as a useful reference to determine the invasiveness of pituitary adenomas.