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目的探讨燃煤型氟中毒对大鼠海马齿状回星形胶质细胞的影响,为地方性氟中毒引起脑损伤的发病机制提供实验依据。方法取断乳2周的SD大鼠90只(平均体重91.1 g,80～100 g),按体重随机分为正常对照组、低氟组(3.3mg/kg)、高氟组(106 mg/kg),每组30只,雌雄各半。除对照组食用正常饲料外,其他各组均食用不同配方饲料,复制氟中毒大鼠模型。模型建立后,取海马组织用常规石蜡切片,行胶质原纤维酸性蛋白免疫组化染色,计数海马齿状回GFAP免疫组化染色阳性反应细胞,并用细胞形态学计量方法测量各阳性反应产物的平均光密度;通过透射电镜观察海马齿状回区星形胶质细胞的细微结构。结果氟中毒组大鼠的主要变化有GFAP免疫组化染色阳性细胞明显多于对照组(P<0.05),阳性反应产物平均光密度明显高于对照组(P<0.05)。结论实验结果提示燃煤型氟中毒大鼠可导致其海马齿状回星形胶质细胞增多;氟可导致大鼠神经发育障碍。
Objective To investigate the effect of fluorosis on the dentate gyrus astrocytes in rats and provide experimental evidence for the pathogenesis of focal fluorosis-induced brain injury. Methods Totally 90 Sprague-Dawley rats (average body weight 91.1 g, 80-100 g) were randomly divided into normal control group, low-fluoride group (3.3 mg / kg) and high-fluoride group (106 mg / kg), each group of 30, male and female. In addition to the normal control group of normal diet, the other groups were fed different formula feed, replicate fluoride poisoning rat model. After establishment of the model, normal paraffin sections of hippocampus were taken for immunohistochemical staining of glial fibrillary acidic protein, and positive cells of hippocampal dentate gyrus were counted by immunohistochemical staining of GFAP. The expression of each positive reaction product Mean optical density. Transmission electron microscopy was used to observe the ultrastructure of astrocytes in dentate gyrus. Results The main changes in rats with fluorosis were GFAP immunohistochemical staining cells were significantly more than the control group (P <0.05), the average optical density of positive reaction products was significantly higher than the control group (P <0.05). Conclusions The experimental results suggest that rats with fluorosis induced by coal-based fluorosis may cause an increase of astrocytes in the dentate gyrus of hippocampus. Fluoride may cause neurodevelopmental disorders in rats.