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通过无患子科荔枝、龙眼,天南星科白鹤芋、火鹤芋、花叶芋,芭蕉科香蕉,葫芦科西瓜、甜瓜,兰科卡特兰、大花蕙兰,石竹科满天星、康乃馨等50多个品种品系体细胞胚胎发生的诱导技术研究,探索出园艺植物获得体细胞发生的两种途径。第一种途径(间接发生途径)由外植体先诱导出愈伤组织然后产生胚状体,此途径的技术关键在于前期必需在附加高浓度2,4-D的培养基上作激发培养,如荔枝、满天星、康乃馨、火鹤芋、香芋等可由此途径产生体胚。第二种途径(直接发生途径)由外值体直接产生体细胞胚胎,此途径的技术关键在于培养基中配合使用较高浓度的6-BA和较低浓度的NAA,由此途径诱导产生体细胞胚胎的园艺植物有香蕉、白鹤芋、卡特兰、大花蕙兰等。
Through the Sapindaceae litchi, longan, Araceae white crane taro, fire taro, taro, banana banana, Cucurbitaceae melon, melon, orchid Catharanthes, Cymbidium, Caryophyllus gypsophila, carnations, etc. More than 50 varieties of lines of somatic embryogenesis induction technology to explore horticultural plants to obtain somatic cells occur in two ways. The first pathway (indirect pathway) is that the explants first induce callus and then produce embryoid bodies. The key point of this approach is that it must be stimulated and cultured on the medium supplemented with 2,4-D at a high concentration in the early stage, Such as lychee, gypsophila, Carnation, Fire Taro, Taro, etc. can be generated by this way somatic embryos. The second pathway (direct pathway) is the direct generation of somatic embryos from outer bodies. The key technology of this pathway lies in that medium used in combination with higher concentration of 6-BA and lower concentration of NAA leads to the production of somatic embryos Horticultural plants of cell embryos are bananas, white taro, Cattleya, Cymbidium and so on.