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1965年澳大利亚抗原被提出后,流行病学、病理学和病毒学材料提示持续性HBV 感染可能是HCC 的发病原因。本文详述一例HCC 临床、病理和电镜检查及组织化学染色结果。材料和方法光镜:死后24小时取材于癌、癌旁及癌肝交界之组织,10%福尔马林固定,石蜡包埋,切片5μm 厚,苏木苏-伊红染色。电镜以伴缓冲液的1%钨酸四氧化物溶液固定1mm(?)大小之癌及癌旁肝组织2小时,于不同浓度之酒精中逐步脱水,包埋于Epon618,用IKB 超薄切片机切片,并以101电镜观察,
After the Australian antigen was introduced in 1965, epidemiological, pathological and virological materials suggested that persistent HBV infection may be the cause of HCC. This article details one clinical, pathological and electron microscopic examination and histochemical staining results of HCC. MATERIALS AND METHODS Light microscopy: Tissues derived from the borders of carcinoma, paraneoplastic, and liver at 24 hours after death were fixed in 10% formalin, embedded in paraffin, sectioned 5 μm thick, and stained with hematoxylin-eosin. Electron microscopy was performed on 1 mm (?) sized tungstic acid tetraoxide solution to fix 1 mm (?) size cancer and adjacent liver tissue for 2 hours, dehydrated in different concentrations of alcohol and embedded in Epon 618, using an IKB ultramicrotome Slicing and observing with 101 electron microscopes,