论文部分内容阅读
目的 :研究McAb共刺激PBLs前后的表型变化及与肝癌细胞凋亡的相关性。方法 :采用流式细胞仪分析PBLs被刺激前后的表型 ,用电镜超微结构和DNA梯子电泳观察肝癌细胞凋亡。结果 :CD3、CD4分子表达高于刺激前约 10 % ,肝癌细胞出现凋亡 ,细胞核固缩、边聚、裂解。同时也出现典型的DNA梯子。而肝癌患者用共刺激PBLs后 ,CD3和CD8增高 (P <0 .0 5 )。结论 :共刺激使共刺激信号分子增加 ,抗原呈递能力增强 ,也使细胞因子分泌增加 ,这些因素促使肝癌细胞凋亡 ,而肿瘤患者T细胞增加时向CD8+ 分化 ,结果提示膜分子的表达与凋亡密切相关
Objective: To study the phenotypic changes of McAb before and after co-stimulation of PBLs and their correlation with apoptosis of hepatocellular carcinoma cells. Methods: The phenotypes of PBLs before and after stimulation were analyzed by flow cytometry. Apoptosis of hepatoma cells was observed by electron microscopy and DNA ladder electrophoresis. RESULTS: The expression of CD3 and CD4 molecules was higher than that of about 10% before the stimulation. Hepatoma cells showed apoptosis, nucleus pyknosis, edge aggregation, and lysis. A typical DNA ladder also appears. In patients with hepatocellular carcinoma, after co-stimulating PBLs, CD3 and CD8 increased (P < 0.05). Conclusion: Co-stimulation increases the number of co-stimulatory signal molecules, enhances the ability of antigen presentation, and also increases the secretion of cytokines. These factors promote the apoptosis of liver cancer cells, and the differentiation of T cells in cancer patients to CD8+, suggesting that the expression and decline of membrane molecules Death is closely related