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利用小麦的600对SSR、391对EST-SSR、35对STS和149对PLUG引物分别对普通小麦品种YN001和十倍体长穗偃麦草基因组DNA进行扩增,旨在分析不同标记在长穗偃麦草的通用性。398(66.3%)对SSR、294(75.2%)对EST-SSR、33(94.3%)对STS和126(84.6%)对PLUG引物在长穗偃麦草有清晰的扩增条带,4种类型引物在长穗偃麦草和小麦间表现多态扩增的引物比例分别为61.0%、68.0%、82.9%和79.9%,表明这4种引物均可以用于长穗偃麦草的遗传研究,但STS和PLUG引物效果优于SSR和EST-SSR。进一步分析基因组SSR和EST-SSR引物的核心单元组成,发现二者在长穗偃麦草有效扩增的引物以二核苷酸和三核苷酸重复为主要类型。
The wheat genomic DNA was amplified from 600 SSR pairs, 391 pairs of EST-SSRs, 35 pairs of STSs and 149 pairs of PLUG primers, respectively, to analyze the genomic DNA of common wheat varieties YN001 and Wheat grass versatility. 398 (66.3%) pairs of SSRs, 294 (75.2%) pairs of EST-SSRs, 33 (94.3%) pairs of STSs and 126 (84.6%) pairs of PLUG primers had a clear amplification band in P. elongata, The primers with the polymorphic amplifications of 61%, 68.0%, 82.9% and 79.9%, respectively, showed that these four primers could be used in the genetic studies of S. elongata, but STS And PLUG primers better than SSR and EST-SSR. Further analysis of the core unit composition of the genomic SSR and EST-SSR primers found that the two primers amplified efficiently dinucleotide and trinucleotide repeats as the main type.