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目的 研究幽门螺杆菌 (Hp)相关性胃肠疾病时胃粘膜组织IL 8、TNF α的活性水平及其病理作用。方法 采用酶联免疫吸附试验夹心法检测胃粘膜组织中IL 8、TNF α的水平 ,并观察IL 8、TNF α水平与胃粘膜炎症活动的关系。结果 1、Hp感染的胃粘膜组织中 ,IL 8或TNF α的浓度明显高于非Hp感染患者。 2、IL 8、TNF α在Hp相关性胃炎与十二指肠球溃疡患者胃粘膜组织的表达无显著性差异。 3、在Hp感染患者中 ,有炎症活动的患者其胃粘膜组织中IL 8、TNF α的表达明显高于无炎症活动的患者。4、Hp感染患者胃粘膜组织中IL 8的表达与TNF α水平呈正相关。结论 炎性细胞因子IL 8、TNF α在Hp相关性胃十二指肠疾病的发病过程中可能起致病作用
Objective To study the activity of IL-8 and TNF-α in gastric mucosa associated with Helicobacter pylori (Hp) -related gastrointestinal diseases and their pathological effects. Methods The levels of IL-8 and TNF-α in gastric mucosa were detected by enzyme-linked immunosorbent assay (ELISA). The relationship between IL-8 and TNF-α levels and gastric mucosal inflammation was also observed. Results 1, Hp-infected gastric mucosa, IL 8 or TNF α concentrations were significantly higher than non-Hp infection. 2, IL-8 and TNF-α in gastric mucosa between Hp-related gastritis and duodenal ulcer patients were not significantly different. In patients with Hp infection, the expression of IL-8 and TNF-alpha in gastric mucosa of patients with inflammatory activity was significantly higher than those without inflammatory activity. The expression of IL-8 in gastric mucosa of patients with Hp infection was positively correlated with the levels of TNF-α. Conclusion The inflammatory cytokines IL 8 and TNF α may play a pathogenic role in the pathogenesis of Hp-related gastroduodenal diseases