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目的探讨GSTT1、GSTM1、NQO1、RAD51和XRCC3基因多态性与我国慢性粒细胞白血病(CML)发生遗传易感之间的关系。方法共120例CML患者和458名与患者无血缘关系的正常人,用多重PCR方法检测GSTT1和GSTM1基因型,用PCR-RFLP方法分析RAD51,XRCC3,NQO1基因型。结果CML患者GSTT1和GSTM1缺失型比例分别为50.8%和59.2%,与正常对照组无显著差异(分别为42.8%和53.1%)。CML患者NQO1C/T和T/T基因型的比例(60.0%)、RAD51G135CG/C基因型比例(26.9%)和XRCC3-241Met杂合子缺失型(Thr/Met)的比例(9.2%)均与正常对照组(分别为65.3%,12.4%和9.2%)无统计学差异。结论本研究结果提示GSTT1、GSTM1、NQO1、RAD51和XRCC3基因型与我国CML的发生无显著相关性。
Objective To investigate the relationship between genetic polymorphisms of GSTT1, GSTM1, NQO1, RAD51 and XRCC3 and genetic susceptibility to chronic myeloid leukemia (CML) in China. METHODS: A total of 120 CML patients and 458 healthy non-related patients were enrolled. The genotypes of GSTT1 and GSTM1 were detected by multiplex PCR and the genotypes of RAD51, XRCC3 and NQO1 were analyzed by PCR-RFLP. Results The proportions of GSTT1 and GSTM1 deletion in CML patients were 50.8% and 59.2%, respectively, which were not significantly different from those in normal controls (42.8% and 53.1%, respectively). The proportion of NQO1C / T and T / T genotypes (60.0%), the ratio of RAD51G135CG / C genotype (26.9%) and XRCC3-241Met heterozygous deletion type (Thr / Met) in CML patients (9.2% The control group (65.3%, 12.4% and 9.2% respectively) showed no significant difference. Conclusion The results of this study suggest that the genotypes of GSTT1, GSTM1, NQO1, RAD51 and XRCC3 have no significant correlation with the occurrence of CML in China.