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采用反相高效液相色谱柱前衍生法测定兔脑匀浆液中氨基酸的含量。衍生试剂是异硫氰酸苯酯（ＰＩＴＣ）。１７种氨基酸衍生物在ＵｃｔｒｏｓｐｈｅｒｅＣ１８柱上经０．０５ｍｏｌ／Ｌ醋酸铵（ｐＨ６．６７）和乙腈的梯度淋洗而分离。包括兴奋性氨基酸递质Ａｓｐ，Ｇｌｕ，ＧＡＢＡ在内的８种氨基酸在２０～３０８ｍｍｏｌ／Ｌ的范围内线性关系良好。保留时间和峰面积的变异系数分别小于２％和０．５％．方法准确，重现性好。定量测定了兔脑中含量较高的上述８种氨基酸
The content of amino acids in homogenate of rabbit brain was determined by RP-HPLC. The derivatizing agent is phenyl isothiocyanate (PITC). Seventeen amino acid derivatives were separated on a Uctrosphere C18 column by a gradient of 0.05 mol / L ammonium acetate (pH 6.67) and acetonitrile. The eight kinds of amino acids including excitatory amino acid transmitters Asp, Glu and GABA had good linearity in the range of 20 ~ 308mmol / L. The coefficient of variation of retention time and peak area were less than 2% and 0.5%, respectively. The method is accurate and reproducible. Quantitative determination of the rabbit brain content of the above eight kinds of amino acids