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目的:探讨β-细辛醚对抑郁模型大鼠生物钟基因(clock)表达的影响。方法:将雄性SD大鼠随机分为正常组,模型组,agomelatine(褪黑素药物激动剂)组,β-细辛醚低、高剂量组5组,每组10只。给予28 d慢性不可预见性刺激(CMUS),复制大鼠抑郁模型。第8天开始模型组、agomelatnie组、β-细辛醚组分别给予生理盐水,agomelatnie(40 mg·kg-1·d-1),β-细辛醚低、高剂量组(12.5,25 mg·kg-1·d-1),5 m L·kg-1,ig。于给药前及第28天分别检测大鼠体重、水平活动次数及糖水偏爱度,评价大鼠行为学改变。处死大鼠冰上取脑,利用实时PCR及,Western blot方法,检测生物钟基因在各组大鼠脑中表达。结果:在行为学方面,模型组大鼠28 d后体重的增加程度,水平活动次数及糖水偏爱度均显著低于正常组及给药组。在基因表达方面,与正常组相比较,模型组clock的表达显著高于正常组,agomelatnin组,β-细辛醚2个剂量组clock的表达无显著差别;与模型组相比较,agomelatine组,β-细辛醚两剂量组clock的表达显著低于模型组。结论:β-细辛醚可能通过影响抑郁大鼠生物钟基因clock在脑中的表达改变大鼠的抑郁状态。
Objective: To investigate the effect of β-asarone on the clock expression in the circadian clock of depression model rats. Methods: Male Sprague-Dawley rats were randomly divided into normal group, model group, agomelatine group, low and high dose β-asarone group, 10 rats in each group. A 28-day chronic unpredictable stimulus (CMUS) was given and a rat model of depression was reproduced. The rats in model group, agomelatnie group and β-asarone group were given normal saline, agomelatnie (40 mg · kg-1 · d-1), low dose of β-asarone · Kg -1 · d -1), 5 m L · kg -1, ig. The body weight, level of activity and preference of syrup were detected before administration and on the 28th day respectively to evaluate the behavioral changes in rats. Rats were sacrificed on ice to take brain, using real-time PCR and Western blot method to detect the expression of circadian clock gene in each rat brain. Results: In behavioral science, the increase of body weight, the number of horizontal activities and the preference of sugar-water in model group rats after 28 days were significantly lower than those in normal group and administration group. In gene expression, compared with normal group, the expression of clock in model group was significantly higher than that in normal group. The expression of clock in agomelatnin group and β-asarone two groups had no significant difference. Compared with model group, agomelatine group, β-asarone two-dose group clock expression was significantly lower than the model group. CONCLUSION: β-asarone may alter the depressive state of rats by affecting the expression of circadian clock in the brain of depressed rats.