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目的 探讨p16、bcl 2、bax蛋白在脑星形细胞瘤中的表达及其意义。方法 采用S P免疫组织化学法 ,检测p16、bcl 2、bax蛋白在 60例不同病理分级脑星形细胞瘤组织中的表达情况。结果 60例脑星形细胞瘤组织中 ,p16、bcl 2、bax蛋白表达的阳性率分别为 5 0 .0 % ( 30 /60 )、5 8.3% ( 35 /60 )、66.7% ( 40 /60 ) ,其中 p16蛋白阳性表达与病理分级有显著性相关 (P <0 .0 5 ) ;bcl 2、bax蛋白表达与病理分级无相关性 (P >0 .0 5 )。 60例脑星形细胞瘤组织中 ,p16、bcl 2、bax蛋白共同表达率 :Ⅰ级为 47.8% ( 11/2 3) ,Ⅲ～Ⅳ级为 0 ( 0 /16) ;在bcl 2、bax蛋白表达的基础上 ,不同病理分级组织中 p16蛋白的缺失率 :Ⅰ级为 4.3% ( 1/2 3) ,Ⅲ～Ⅳ级为 5 0 .0 % ( 8/16) ,有非常显著性差异 (P <0 .0 1)。结论 p16蛋白表达与脑星形细胞瘤的发生发展密切相关 ;在脑星形细胞瘤的发展过程中 ,bcl 2、bax、p16蛋白可能有某种协调作用。
Objective To investigate the expression of p16, bcl2 and bax in brain astrocytoma and its significance. Methods SP immunohistochemical method was used to detect the expression of p16, bcl2 and bax protein in 60 cases of pathologically graded astrocytomas. Results The positive rates of p16, bcl2 and bax in 60 astrocytomas were 50.0% (30/60), 35.3% (35/60) and 66.7% (40/60), respectively ). There was a significant correlation between the positive expression of p16 protein and pathological grade (P <0.05). There was no correlation between the expression of bcl-2 and bax proteins and pathological grade (P> 0.05). The expression rates of p16, bcl2 and bax protein in 60 cases of astrocytomas were 47.8% (Ⅰ / 2/3) and 0 (1/16) for Ⅲ ~ Ⅳ, respectively. The expressions of bcl 2, bax On the basis of protein expression, the deletion rate of p16 protein in different pathological grading tissues was 4.3% (1/2 3) in grade Ⅰ and 50.0% (8/16) in grade Ⅲ-Ⅳ, with significant difference (P <0. 01). Conclusion The expression of p16 protein is closely related to the development of astrocytoma. In the development of astrocytomas, bcl 2, bax and p16 proteins may have some coordination roles.