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将肌红蛋白(Mb)通过吸附的方法固定在碳纳米管(CNT)表面,用AFM、XPS、UV-Vis和FTIR对其进行了表征,研究了CNT对Mb直接电子转移反应的促进作用.循环伏安结果表明,Mb在CNT表面能进行有效和稳定的直接电子转移反应,其循环伏安曲线上表现出一对良好的、几乎对称的氧化还原峰;在20-160mV·s-1的扫速范围内,式量电位E0′几乎不随扫速而变化,其平均值为(-0.343±0.001)V(vsSCE,pH7.0);Mb在CNT表面直接电子转移的表观速率常数为(3.11±0.98)s-1;式量电位E0′与溶液pH的关系表明,Mb的直接电化学过程是一个有H+参与的电极过程.进一步的实验结果显示,固定在CNT表面的Mb能保持其对H2O2和O2还原的生物电催化活性.
The myoglobin (Mb) was immobilized on the surface of carbon nanotubes (CNTs) by adsorption and characterized by AFM, XPS, UV-Vis and FTIR. The promoting effect of CNT on Mb direct electron transfer reaction was studied. The results of cyclic voltammetry showed that Mb could efficiently and stably direct electron transfer reaction on the CNT surface, and showed a good and almost symmetric redox peak on the cyclic voltammogram. At 20-160mV · s-1 The sweeping speed range, the type potential E0 ’almost does not change with the sweep rate, the average value of (vsSCE, pH7.0); Mb in the CNT surface of the direct electron transfer apparent rate constant ( 3.11 ± 0.98) s-1. The relationship between E0 ’and solution pH indicates that the direct electrochemical process of Mb is an electrode process with H + participation.Further experimental results show that Mb immobilized on the CNTs can maintain its Bioelectrocatalytic Activity for H2O2 and O2 Reduction.