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目的 :观察不同浓度的胶质细胞源性神经营养因子 (GDNF) ,对大鼠胚胎脊髓运动神经元生长活性的作用。方法 :取大鼠胚胎脊髓腹侧组织体外分离 ,进行原代细胞培养 ,应用抗神经微丝单克隆抗体 (mAb)SMI32进行运动神经元的免疫细胞化学染色 ,从细胞形态学及应用MTT比色法 ,研究GDNF对大鼠脊髓运动神经元的影响。结果 :GDNF能明显促进体外培养的大鼠脊髓运动神经元存活及突起的生长 (P <0 .0 5 ) ,且具有剂量依赖的趋势。结论 :不同浓度的GDNF对体外培养的大鼠胚胎脊髓运动神经元 ,有不同程度的促生长作用
Objective: To observe the effects of different concentrations of glial cell line-derived neurotrophic factor (GDNF) on the growth activity of rat spinal cord motoneurons. Methods: Ventral spinal cord tissue of rat embryos was isolated and cultured in vitro. Immunocytochemical staining of motor neurons was performed using anti-neurofilament monoclonal antibody (mAb) SMI32. Cell morphology and MTT colorimetric assay Method to study the effect of GDNF on spinal motoneurons in rats. Results: GDNF significantly promoted the survival and neurite outgrowth of rat spinal cord motor neurons (P <0.05) in a dose-dependent manner. Conclusion: Different concentrations of GDNF can promote the growth of rat embryonic spinal cord motor neurons in vitro