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目的 探讨醋酸铅 (PbAc)对大鼠脑细胞凋亡的诱发作用及对bcl 2基因表达的影响。方法 SD大鼠腹腔分别注射PbAc 2 5、5 0、10 0mg/kg 5d ,第 6天用流式细胞仪分别测定其大脑皮层、海马和小脑组织的细胞凋亡率和bcl 2基因的表达产物Bcl 2蛋白含量。结果 染铅大鼠大脑皮层、海马、小脑细胞凋亡率 (% ) :2 5mg/kgPbAc组为 5 .80± 0 .87、4.82± 0 .37、4.82± 1.2 3 ,5 0 mg/kg组值高于 2 5mg/kg组 ,10 0mg/kg组值高于 5 0mg/kg组 ,均比对照组 (1.40± 0 .70、2 .0 0± 0 .6 3、1.6 6±0 .49)高 ,差异有显著性 (P <0 .0 1) ;并有剂量 -反应关系 (r值分别为 0 .998、0 .989、0 .997)。染铅大鼠大脑皮层、海马、小脑bcl 2基因表达 (FI指数 ) :2 5mg/kgPbAc组为 0 .6 8± 0 .0 3、0 .6 1± 0 .0 6、0 .6 9± 0 .0 5 ;5 0mg/kg、10 0mg/kg组值均与 2 5mg/kg组接近 ,但均比对照组 (1.0 0± 0 .13、1.0 0±0 .17、1.0 0± 0 .13)降低 ,差异有显著性 (P <0 .0 1) ;并有剂量 -反应关系 (r值分别为 0 .886、0 .787、0 .832 )。大鼠大脑皮层、海马和小脑细胞凋亡率与Bcl 2含量呈负相关 (r值分别为 - 0 .75 0、- 0 .5 0 9、- 0 .6 6 7)。结论 铅可以诱发大鼠脑细胞凋亡 ,其机制可能与脑组织内bcl 2基因表达下调有关
Objective To investigate the effects of lead acetate (PbAc) on the apoptosis of rat brain cells and its effect on bcl 2 gene expression. Methods Sprague-Dawley rats were injected intraperitoneally with PbAc 2, 5, 10 and 10 mg / kg for 5 days respectively. The apoptosis rate and the expression of bcl 2 gene in cerebral cortex, hippocampus and cerebellum were measured by flow cytometry Bcl 2 protein content. Results The apoptotic rate (%) in cerebral cortex, hippocampus and cerebellum of lead-exposed rats was 5.80 ± 0.87,4.82 ± 0.37,4.82 ± 1.2 3 and 50 mg / kg in 25 mg / kg PbAc group Values higher than 25mg / kg group, 10 0mg / kg group higher than the 50mg / kg group than the control group (1.40 ± 0.70,2 .0 0 ± 0 .6 3,1.6 6 ± 0.49 ), The difference was significant (P <0. 01); and dose-response relationship (r values were 0.998, 0.989, 0.997). The expression of bcl-2 mRNA in the cerebral cortex, hippocampus and cerebellum of lead-exposed rats: (FI index) 25 mg / kg PbAc group was 0.68 ± 0.30, 0.6 ± 0.6,0.69 ± The values of 0. 05; 5 0 mg / kg and 10 0 mg / kg were all close to those of 25 mg / kg group, but both were higher than those of the control group (1.0 0 ± 0.13, 1.0 ± 0.17, 1.0 ± 0. 13), the difference was significant (P <0.01); and dose-response relationship (r values were 0.886,0 .787,0. 832). There was a negative correlation between the apoptosis rate of cerebral cortex, hippocampus and cerebellum and the content of Bcl 2 in rats (r = -0.75 0, -0.059, -0.667, respectively). Conclusion Lead can induce the apoptosis of rat brain cells, which may be related to the down-regulation of bcl 2 gene expression in brain tissue