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目的:研究Survivin、Bcl-2及nm23-H1的表达及其与鼻咽癌(NPC)病理类型、临床分期之间的关系。方法:应用免疫组化分析32例NPCSurvivin、Bcl-2及nm23-H1的表达。结果:32例NPC组织中Sur-vivin、Bcl-2及nm23-HI的表达率分别为:68.7%(22/32)、71.9%(23/32)、56.3%(18/32);晚期(Ⅲ、Ⅳ期)患者Survivin、Bcl-2阳性表达率分别为90%(18/20)、90%(18/20)明显高于早期(Ⅰ、Ⅱ期)患者的表达率33.3%(4/12)、41.6%(5/12),差异均有显著性(P<0.05);未分化癌患者Survivin、Bcl-2阳性表达率分别为100%(10/10)、100%(10/10)明显高于低分化鳞癌患者表达率54.5%(12/22)、59.1%(13/22),差异均有显著性(P<0.05);有颈部淋巴转移的患者Survivin、Bcl-2阳性表达率分别为69.2%(18/26)、76.9%(20/26)高于无颈部淋巴转移患者表达率66.6%(4/6)、50%(3/6),但均未见显著性(P>0.05);晚期(Ⅲ、Ⅳ期)患者nm23-H的阳性表达率40%(8/20)低于早期(Ⅰ、Ⅱ期)患者的表达水平100%(12/12),差异有显著性(P<0.05),未分化癌患者的阳性表达率(7/10,70%)高于低分化鳞癌患者的表达率54.5%(13/22),但差异无显著性(P>0.05),无颈部淋巴转移患者nm23-H1的阳性表达率100%(6/6)高于有颈部淋巴转移患者表达水平46.1%(12/26),差异有显著性(P<0.05)。结论:鼻咽癌中存在着Survivin、Bcl-2及nm23-H1的高表达;Survivin、Bcl-2与鼻咽癌的临床分期、病理类型具有相关性,而与颈部淋巴转移无相关性,nm23-H1与鼻咽癌的临床分期、颈部淋巴转移具有相关性,但与病理类型无相关性。
OBJECTIVE: To study the expression of Survivin, Bcl-2 and nm23-H1 and its relationship with the pathological type and clinical stage of nasopharyngeal carcinoma (NPC). Methods: Immunohistochemistry was used to analyze the expression of NPCSurvivin, Bcl-2 and nm23-H1 in 32 cases. Results: The expression rates of Survivin, Bcl-2 and nm23-HI in 32 NPC tissues were 68.7% (22/32), 71.9% (23/32) and 56.3% (18/32) The positive rates of Survivin and Bcl-2 were 90% (18/20) and 90% (18/20) in patients with stage Ⅲ and Ⅳ, respectively. The positive rate of Survivin and Bcl- 12, 41.6% (5/12) respectively. The positive rates of Survivin and Bcl-2 were 100% (10/10) and 100% ) Were significantly higher than those in poorly differentiated squamous cell carcinoma (54.5% (12/22), 59.1% (13/22), respectively) (P <0.05). The expressions of Survivin and Bcl-2 in patients with cervical lymphatic metastasis The positive rates were 69.2% (18/26), 76.9% (20/26) respectively, and 66.6% (4/6) and 50% (3/6) of those without cervical lymph node metastasis, respectively The positive rate of nm23-H was 40% (8/20) in the advanced stage (stage Ⅲ, Ⅳ), which was lower than that in the early stage (stage Ⅰ, Ⅱ) , The difference was significant (P <0.05). The positive expression rate in undifferentiated carcinoma (7/10, 70%) was higher than that in poorly differentiated squamous cell carcinoma (54.5%, 13/22), but there was no significant difference (P> 0.05), the positive rate of nm23-H1 in patients with cervical lymph node metastasis The expression rate of 100% (6/6) than with cervical lymph node metastasis expression levels were 46.1% (12/26), there was a significant (P <0.05) difference. CONCLUSIONS: Survivin, Bcl-2 and nm23-H1 are highly expressed in NPC. Survivin and Bcl-2 are correlated with the clinical stages and pathological types of NPC, but not with lymph node metastasis, nm23-H1 and nasopharyngeal carcinoma clinical stage, cervical lymph node metastasis, but no correlation with pathological types.