我们应用破伤风类毒素体外免疫的人扁桃体细胞和人-鼠异源骨髓瘤RF 系细胞进行融合,从中筛选到一株杂交瘤细胞89112—50,并通过克隆化筛选获得了两个亚克隆,其分泌的抗体是抗原特异的,和三种抗原(OVA、TC-S、FYG)都不交叉,分泌抗体的功能也比较稳定,在培养瓶内连续扩增传代13次后,仍维持相当高的抗体分泌能力,在常规传代培养过程中所收集的培养物上清液中抗体的含量平均为69.6μg/ml。 We use tetanus toxoid in vitro immunized human tonsillar cells and human - mouse heterologous myeloma RF line cells were fused to select a hybridoma cells 89112-50, and obtained by cloning two subclones, The secreted antibodies are antigen-specific, and do not cross with the three antigens (OVA, TC-S, FYG). The function of secreting antibodies is also relatively stable. After continuously passaged in culture flasks for 13 passages, the antibodies remained relatively high The antibody-secreting capacity of the culture supernatant collected during the conventional subculture was 69.6 μg / ml on average.