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目的:探讨“冬病夏治”方药配伍对延胡索乙素经皮吸收特性的影响。方法:采用Franz扩散池和离体大鼠皮肤进行体外透皮试验,利用HPLC测定体外透皮接收液中延胡索乙素的含量,流动相甲醇-0.1%甲酸,检测波长280 nm,流速0.8 m L·min-1。计算不同方药配伍的“冬病夏治”贴膏中延胡索乙素的累积渗透量和渗透速率。结果:不同方药配伍的“冬病夏治”贴膏中延胡索乙素体外透皮行为均符合零级动力学方程,全方组的透皮速率(1.04μg·cm-2·h-1)和24 h累积渗透量(29.54μg·cm-2)均大于单味药,差异具有极显著性。全方组与缺甘遂和缺细辛组相比,延胡索乙素的体外渗透率无显著性差异,但与缺白芥子组相比具有显著性差异。结论:“冬病夏治”方药配伍对延胡索乙素的经皮渗透特性有一定影响,方中白芥子能促进延胡索乙素的经皮吸收。
Objective: To investigate the effect of compatibility of “Winter disease Xiazhi” prescription on the percutaneous absorption of tetrahydropalmatine. METHODS: In vitro skin permeation experiments were performed using Franz diffusion cells and isolated rat skin. The content of tetrahydropalmatine in the transdermal receiving solution was measured by HPLC. The mobile phase was methanol-0.1% formic acid, detection wavelength was 280 nm, flow rate was 0.8 m L. ·min-1. Calculate the cumulative penetration and penetration rate of tetrahydropalmatine in “Winter Disease Xiazhi” patch with different prescriptions. RESULTS: The in vitro transdermal behavior of tetrahydropalmatine in “Winter Disease Xiazhi” patch with different prescriptions was in accordance with the zero-order kinetic equation, and the transdermal speed of the whole group was 1.04 μg·cm-2·h-1. The cumulative permeation volume (29.54 μg·cm-2) and 24 h were greater than that of single herb, and the difference was extremely significant. There was no significant difference in the in vitro permeability of tetrahydropalmatine between the complete group and the lack of kansui and ganoderma lucidum group, but there was a significant difference compared with the lack of white mustard group. Conclusion: The combination of “Winter disease Xiazhi ” prescription has certain influence on the percutaneous permeability of tetrahydropalmatine. Fang mustard can promote the percutaneous absorption of tetrahydropalmatine.