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目的探讨乙型肝炎病毒(hepatitis B virus,HBV)基因型与肝病转归的关系。方法随机选取中国西部、北部地区汉族HBV感染者DNA含量104以上者410例,煮沸法提取HBV基因组模板,PCR扩增S区基因,应用自动测序仪测定目的基因序列,进行基因分型,应用SPSS10.0软件统计分析。结果C型占91.95%,B型占6.83%,B、C混合型占0.24%,D型占0.98%。疾病进展研究分4种情形对基因型C、基因型B进行单因素方差分析:慢性病毒携带者(ASC),慢性乙型肝炎(chronic hepatitisB,CHB),肝硬化(liver cirrhosis,LC),原发性肝细胞性肝癌(primary hepatocellular carcinoma,HCC)。统计显示,感染基因型C后CHB更易发展为LC和(或)HCC,有统计学意义,但两基因型之间LC发展为HCC差别无统计学意义。LCvs.(CHB+HCC)统计显示:两组在基因型方面虽无统计学差异,但从Means Plots可以看出感染基因型C向肝硬化或肝细胞癌方向发展的趋势更明显。结论中国西部、北部汉族HBV感染者中以感染基因型C为主,且感染基因型C较基因型B更容易发展为严重的肝病。
Objective To investigate the relationship between hepatitis B virus (HBV) genotype and the prognosis of liver disease. Methods A total of 410 Han patients with HBV DNA in the western and northern regions of China were enrolled in this study. 410 cases were genotyped by boiling method, and the S gene was amplified by PCR. The gene sequences were sequenced and sequenced by SPSS10 .0 Software Statistical Analysis. Results C type accounted for 91.95%, B type accounted for 6.83%, B, C mixed accounted for 0.24%, D type accounted for 0.98%. The study of disease progression included four kinds of cases: univariate analysis of variance of genotype C and genotype B: chronic viral hepatitis B (CHB), chronic cirrhosis (CHB), cirrhosis (LC) Primary hepatocellular carcinoma (HCC). Statistics show that CHB is more likely to develop into LC and / or HCC after genotype C infection, with statistical significance, but there is no significant difference in LC development between the two genotypes for HCC. LCvs. (CHB + HCC) statistics showed that there was no significant difference in genotypes between the two groups, but the tendency of infected genotype C to cirrhosis or hepatocellular carcinoma was more obvious from Means Plots. Conclusions Genotype C is predominant among HBV infected Han and Han people in western and northern China, and genotype C is more likely to develop severe liver disease than genotype B.