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目的:建立应用于无花丹参快速繁殖的组织培养体系。方法:以茎尖为材料,优化其脱毒培养和植株再生的激素浓度组合,筛选出最佳组合。结果:在添加适宜浓度6-BA(1.5 mg/L)和NAA(0.1 mg/L)的MS培养基上能有效诱导茎尖的芽发生,诱导率可达90.8%。丹参叶片愈伤组织诱导与继代增殖的适宜培养基为2.0 mg/L 6-BA+1.0 mg/L NAA;最佳分化培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA,芽发生率达到90%;1/2MS+0.5mg/L IBA适宜于试管苗生根。结论:所建立的无花丹参植株再生体系可为解决丹参栽培中的资源和质量问题以及在分子水平进行品种改良打下了良好基础。
Objective: To establish a tissue culture system for the rapid propagation of Salvia miltiorrhiza. Methods: The best combinations were screened out based on the combination of hormone concentrations in shoot tips and detoxification and plant regeneration. Results: Buds of shoot tips were effectively induced on MS medium supplemented with appropriate concentrations of 6-BA (1.5 mg / L) and NAA (0.1 mg / L) with the induction rate of 90.8%. The optimal culture medium for the induction and subculture of Salvia miltiorrhiza leaves was 2.0 mg / L 6-BA + 1.0 mg / L NAA. The best differentiation medium was MS + 1.0 mg / L 6-BA + 0.5 mg / L NAA , Bud incidence reached 90%; 1 / 2MS + 0.5mg / L IBA suitable for rooting of plantlets. Conclusion: The establishment of Salvia miltiorrhiza plant regeneration system can lay a good foundation for the solution of Salvia miltiorrhiza resources and quality problems and the improvement of varieties at the molecular level.