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测定了栉孔扇贝幼贝在不同浓度Cu2+胁迫下体内酸性磷酸酶(ACP)和碱性磷酸酶(AKP)的活力变化以及过氧化氢(H2O2)含量变化情况。结果表明,当Cu2+浓度为0.01mg·L-1时幼贝体内ACP和AKP活力明显高于对照组,且差异极显著(p<0.01),随Cu2+浓度的增加,酶活力逐渐呈下降趋势,在0.1mg·L-1时酶活力低于对照组。扇贝幼贝H2O2含量的变化在0.01mg·L-1和0.02mg·L-1的低浓度Cu2+影响下呈缓慢升高的趋势,但总体变化不明显,而当Cu2+浓度达到0.05mg·L-1时,H2O2含量明显高于对照组,且差异极显著(p<0.01),随Cu2+浓度的增加,H2O2含量继续升高。以上数据表明Cu2+对栉孔扇贝幼贝机体的免疫防御功能有明显影响。在海洋污染中,可能是使扇贝幼苗抗病力减弱、种质退化、并引起大量死亡的一个较为重要的因素。
The changes of acid phosphatase (ACP) and alkaline phosphatase (AKP) activity and the content of hydrogen peroxide (H2O2) in the scallop shell of Chlamys farreri were determined under different concentrations of Cu2 + stress. The results showed that when the concentration of Cu2 + was 0.01 mg · L-1, the activity of ACP and AKP was significantly higher than that of the control group (P <0.01). With the increase of Cu2 + concentration, the activity of ACP and AKP decreased gradually, At 0.1 mg · L-1 enzyme activity was lower than the control group. The changes of H2O2 contents in the scallop shell showed a slowly increasing trend under the influence of Cu2 + of 0.01 mg · L-1 and 0.02 mg · L-1, but the overall change was not obvious. When the concentration of Cu2 + reached 0.05 mg · L- 1, H2O2 content was significantly higher than the control group, and the difference was significant (p <0.01), with the increase of Cu2 + concentration, H2O2 content continued to rise. These data indicate that Cu2 + has a significant effect on the immune defensive function of the juvenile of Chlamys farreri. In marine pollution, it may be one of the more important factors for reducing the disease resistance, degenerating germplasm and causing a large number of death in scallop seedlings.