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以直链淀粉含量 ( AC)中等的 CT9993和泰国优质的香软米 KDML10 5杂交产生的 152个重组近交系 ( RIL )为材料 ,构建了含 83个 RFLP、 69个 AFL P和 15个微卫星 ( SSL P)标记的分子标记连锁图 ,标记间平均距离为 12 .98c M。应用该连锁图对控制稻米 AC的基因座位 ( QTL )进行了分析。结果表明 :稻米 AC主要受两个主效 QTL和 5个微效 QTL的共同控制 ,两个主效 QTL分别位于第 3和第6染色体上 ,它们分别与 R2 170和 R1962距离 10 .5c M和 3.6c M,其中 R1962与 wx基因紧密连锁。上述两个位点都以大于 8.0的 L OD值检测到。根据分析结果 ,初步推断这两个主效 QTL无互作。文中还讨论了在偏态群体中连锁作图和 QTL定位的注意点
A total of 152 recombinant inbred lines (RILs) derived from a cross between CT9993 with amylose content (AC) and Thai high quality KDML10 5 were used as materials to construct 83 RFLPs, 69 AFLPs and 15 microsatellites Satellite (SSL P) marker molecular marker linkage map, the average distance between the markers was 12.98cM. The linkage map was used to analyze the genetic locus (QTL) controlling rice AC. The results showed that AC was mainly controlled by two main-effect QTLs and five minor QTLs, and the two main-effect QTLs were located on chromosomes 3 and 6, respectively, which were respectively located at 10.5c M and R2 170 and R1962 3.6c M, of which R1962 is closely linked to the wx gene. Both sites were detected with LOD values greater than 8.0. According to the results of the analysis, we conclude that there is no interaction between the two major QTLs. The paper also discusses the attention points of linkage mapping and QTL mapping in skewed population