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油菜品质的改进及价格的提高使得全世界的油菜种植面积迅速扩大。我们(Barsby等,1987)及其他人(Pelletier等,1983)已运用原生质体融合技术进一步研究这一作物,目标是创造具有甘蓝型油菜细胞核细胞质雄性不育源(如波里马和甘蓝型油菜细胞质)的线粒体和对三氯杂苯类除草剂具抗性的白菜型油菜叶绿体的细胞质杂种。从其它可能的细胞器组合中准确地鉴定出所需要的细胞质杂种的唯一方法是建立一种能区分所有可能组合的线粒体DNA和叶绿体DNA的分析体系。
Improvements in rapeseed quality and higher prices have led to a rapid expansion of canola cultivation throughout the world. This crop has been further studied using protoplast fusion techniques by us (Barsby et al., 1987) and others (Pelletier et al., 1983) with the goal of creating cytoplasmic male sterility sources with nucleus of Brassica napus (eg, Polima and Brassica napus Cytoplasm) and cytoplasmic hybrids of chloroplasts in Brassica napus resistant to trichlorobenzene herbicides. The only way to accurately identify the desired cytoplasmic hybrids from other possible combinations of organelles is to establish an assay that separates all possible combinations of mitochondrial DNA and chloroplast DNA.