目的建立酸枣仁的超高效液相指纹图谱。方法采用ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)色谱柱;流动相为乙腈-0.05%磷酸水,梯度洗脱,流速为0.5 mL.min-1,柱温为30℃,检测波长为204 nm。结果建立了酸枣仁的超高效液相色谱指纹图谱共有模式,标定了17个共有峰,并指认了5个主要色谱峰,14批酸枣仁药材的相似度在0.947～0.994之间。结论本方法快速、高效,可用于酸枣仁的质量评价。 Objective To establish ultra-high performance liquid chromatography (HPLC) fingerprints of Spinosae Radix. Methods The column was ACQUITY UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution at a flow rate of 0.5 mL · min-1 and a column temperature of 30 ℃. The detection wavelength 204 nm. Results The common mode of HPLC fingerprint was established, 17 common peaks were calibrated and 5 main chromatographic peaks were identified. The similarity of 14 batches of Zizyphus jujuba was between 0.947 and 0.994. Conclusion The method is rapid and efficient and can be used for the quality evaluation of wild jujube.